Nano-SiO2 is an inexpensive material for enzyme immobilization and has been frequently utilized for this purpose. In addition to its low cost, Nano-SiO2 has several advantages when used as a supporting material, including its lack of toxicity and chemical reactivity, allowing easy fixation of enzymes. In this article, Porcine Pancreatic lipase was non-covalently immobilized on Nano-SiO2. The properties of immobilized enzyme were then defined. The optimum pH and temperature for the immobilized PPL for hydrolysis of olive oil were determined as 7.6 and 40-50 °C, respectively. The immobilized enzyme possessed high stability in batch operation; with 73.5% of the initial activity still remaining after 8 times of repeated operation of the immobilized lipase. The immobilized enzyme was more stable than free enzyme and t1/2 was 25 d, while free lipase activity was lost 50% in 2 days. The apparent Km for the immobilized enzyme was significantly smaller than that of the free one.
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| http://dx.doi.org/10.1166/jnn.2018.15366 | DOI Listing |
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