Clonal propagation plays a critical integral role in the growth and success of a global multi-billion dollar horticulture industry through a constant supply of healthy stock plants. The supply chain depends on continuously improving the micropropagation process, thus, understanding the physiology of in vitro plants remains a core component. We evaluated the influence of exogenously applied cytokinins (CKs, N-benzyladenine = BA, isopentenyladenine = iP, meta-topolin = mT, 6-(3-hydroxybenzylamino)-9-(tetrahydropyran-2-yl)purine = mTTHP) in Murashige and Skoog (MS)-supplemented media on organogenic response and accumulation of endogenous CK and indole-3-acetic acid (IAA) metabolites. The highest shoot proliferation (30 shoots/explant) was obtained with 20 μM mT treatment. However, the best quality regenerants were produced in 10 μM mT treatment. Rooting of Amelanchier alnifolia in vitro plantlets was observed at the lowest CK concentrations, with the highest root proliferation (3 roots/explant) in 1 μM mTTHP regenerants. Similar to the organogenic response, high levels of endogenous bioactive CK metabolites (free bases, ribosides, and nucleotides) were detected in mT and mTTHP-derived regenerants. The level of O-glucosides was also comparatively high in these cultures. All CK-treated plants had high levels of endogenous free IAA compared to the control. This may suggest an influence of CKs on biosynthesis of IAA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.nbt.2018.02.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Underutilized Canadian wild berries as potential sources of lipophilic bioactive compounds with antihypertensive properties.
Food Funct
July 2024
Agriculture and Agri-Food Canada, Morden Research and Development Centre, Morden, Manitoba, R6M 1Y5, Canada.
Traditional berries are small fruits and are widely distributed in the Canadian prairies. The current study investigates the lipophilic bioactive compounds such as fatty acids, phytosterols, and terpenes, and their bioactivities, such as lipid peroxidation, as well as the antihypertensive activities of fourteen underutilized Canadian wild berries. These berries include Saskatoon berries (), gooseberries (), wild grapes (), blackcurrants (), redcurrants (), haskap berries (), wild raspberries (), wild blueberries (), chokeberries (), buckthorn (), highbush cranberries (), chokecherries (), nannyberries () and snowberries ().
View Article and Find Full Text PDFFirst report of causing Saskatoon serviceberry rust in Canada.
Plant Dis
January 2024
The University of British Columbia Department of Forest & Conservation Sciences, 175095, 2424 Main Mall, Vancouver, British Columbia, Canada;
Saskatoon serviceberry (Amelanchier alnifolia) is a tall shrub native to the western regions of North America. In British Columbia, serviceberries are a resource for Indigenous communities, used in cooking and for medicinal purposes (Turner et al. 1990).
View Article and Find Full Text PDFPolyphenolic profiles of a variety of wild berries from the Pacific Northwest region of North America.
Curr Res Food Sci
August 2023
Department of Nutrition and Exercise Physiology, Washington State University, Spokane, WA, USA.
Polyphenols have been extensively profiled and quantified in commercially grown berries, but similar information is sparsely available for wild berries. Because polyphenolic contents are inherently associated with berries health benefits, determining phenolic profiles is an important step for strategizing potential uses by the industry and for health and nutrition outcomes. Here, we profiled phenolic compounds in wild berries commonly encountered and harvested in the Pacific Northwest region of North America.
View Article and Find Full Text PDFMarket potential and value-added opportunities of cold-hardy berries and small fruits in the Intermountain West, USA.
J Food Sci
February 2023
Sustainable Food Systems Program, Department of Health and Human Development, Montana State University, Bozeman, Montana, USA.
Novel cold-hardy berries and small fruits represent an opportunity for growers in the Intermountain West, USA, as the harsh environment is not suited for other common berries and small fruits. This study analyzed the fresh market and value-added potential of haskap berries (Lonicera caerulea), saskatoon berries (Amelenchier alnifolia), and dwarf sour cherry (DSC) fruit (Prunus x kerrasis) by instrumental and consumer studies. Fresh and 2-week stored haskap (cv.
View Article and Find Full Text PDFInfluence of Rosaceous Species and Driving Factors on Differentiation of Rhizospheric Bacteria in a Deciduous Broad-Leaved Forest.
Curr Microbiol
October 2022
School of Ecology and Environment, Anhui Normal University, 189# South Jiuhua Road, Yijiang District, Wuhu, 241002, China.
Understanding plant-microbe interactions could provide the basis for improved phytoremediation, microbial resource utilization, and secondary metabolite production. Rhizosphere bacterial communities are strongly influenced by abiotic factors such as soil nutrient availability and the composition of such communities exhibits differentiation under different host plants. In a deciduous broad-leaved forest in Anhui Province, eastern China, the rhizospheric bacteria of three different tree species of the Rosaceae family (Sorbus alnifolia, Cerasus serrulata, and Photinia beauverdiana) were studied, with the bacteria of the bulk soil as controls.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!