Anal Chim Acta
School of Pharmacy, Faculty of Science, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor, Malaysia. Electronic address:
Published: June 2018
Single strand DNA (ssDNA) chimeras consisting of a silver nanoclusters-nucleating sequence (NC) and an aptamer are widely employed to synthesize functional silver nanoclusters (AgNCs) for sensing purpose. Despite its simplicity, this chimeric-templated AgNCs often leads to undesirable turn-off effect, which may suffer from false positive signals caused by interference. In our effort to elucidate how the relative position of NC and aptamer affects the fluorescence behavior and sensing performance, we systematically formulated these NC and aptamer regions at different position in a DNA chimera. Using adenosine aptamer as a model, we tested the adenosine-induced optical response of each design. We also investigated the effect of linker region connecting NC and aptamer, as well as different NC sequence on the sensing performance. We concluded that locating NC sequence at 5'-end exhibited the best response, with immediate fluorescence enhancement observed over a wide linear range (1-2500 μM). Our experimental findings help to explain the emission behavior and sensing performance of chimeric conjugates of AgNCs, providing an important means to formulate a better aptasensor.
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http://dx.doi.org/10.1016/j.aca.2018.01.012 | DOI Listing |
Biosens Bioelectron
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Biophotonic Nanosensors Laboratory, Centro de Física Aplicada y Tecnología Avanzada (CFATA), Universidad Nacional Autónoma de México (UNAM), Querétaro, 76230, Mexico. Electronic address:
Smartphone-based colorimetric (bio)sensing is a promising alternative to conventional detection equipment for on-site testing, but it is often limited by sensitivity to lighting conditions. These issues are usually avoided using housings with fixed light sources, increasing the cost and complexity of the on-site test, where simplicity, portability, and affordability are a priority. In this study, we demonstrate that careful optimization of color space can significantly boost the performance of smartphone-based colorimetric sensing, enabling housing-free, illumination-invariant detection.
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Ionian Department, University of Bari Aldo Moro, Bari, Italy.
Fugitive or diffuse methane emissions constitute an important source of damage to the environment, much greater even than CO2 both over a time span of 20 years and over a longer time span of 100. It is therefore of preeminent importance to undertake all the efforts necessary to implement new tools, protocols, and methods that contribute to the identification and measurement of these emissions to implement site-specific actions of mitigation, repair, and conscious management of the emitting plants. Among the remote sensing and leak detection technologies currently used, the tunable diode laser absorption spectroscopy (TDLAS) method plays a relevant role.
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National Center for International Research on Green Optoelectronics, Guangdong Provincial Key Laboratory of Optical Information Materials and Technology, Institute of Electronic Paper Displays, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, China.
Ice melting, a common yet complex phenomenon, remains incompletely understood. While theoretical studies suggest that preexisting defects in ice generate "off-lattice" water molecules, triggering bulk ice melting, direct experimental evidence of their form has been lacking as the transparent and transient nature of ice poses significant challenges for observation with current techniques. Here, we introduce an ice-melting-induced lyophilization (IMIL) technique that employs graphene-based nanoprobes to replicate and track liquid evolution within melting bulk ice.
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National Key Laboratory of Uranium Resources Exploration-Mining and Nuclear Remote Sensing, East China University of Technology, Nanchang 330013, China.
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Department of Cardiology Saitama Medical University, International Medical Center Hidaka Japan.
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