Objective: This study aimed to explore the immunoregulatory effect of flavonoids of blueberry ( L.) leaves (FBL).
Methods: The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-Tof-MS). The immunoregulatory effect and possible regulatory mechanisms of FBL were investigated in lipopolysaccharide- (LPS-) induced RAW 264.7 cells.
Results: According to the results of UPLC/Q-Tof-MS, nine flavonoids of blueberry leaves were identified. FBL showed a significant reduction in the production of TNF- in LPS-stimulated RAW 264.7 cells. FBL significantly decreased the expression of NF-B p65 and P-NF-B p65 in LPS-induced RAW 264.7 cells in a dose-dependent manner.
Conclusion: Our study showed the immunoregulatory effect of FBL through the suppression of TNF- via the NF-B signal pathway.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763064 | PMC |
| http://dx.doi.org/10.1155/2017/5476903 | DOI Listing |
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