AI Article Synopsis
- The research focuses on how the arrangement of extracellular matrix (ECM) components influences cell adhesion and differentiation by creating large-scale uneven nanopatterns using RGD-functionalized dendrimers.
- The nanopatterns are achieved through surface adsorption from solutions, and their characteristics are analyzed using techniques like scanning tunneling microscopy and atomic force microscopy.
- The method developed is simple and scalable, making it compatible with cell culture and applicable to other cellular ligands that affect cell behavior based on their concentration.
Article Abstract
Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908668 | PMC |
| http://dx.doi.org/10.3791/56347 | DOI Listing |
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Article Synopsis
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- The nanopatterns are achieved through surface adsorption from solutions, and their characteristics are analyzed using techniques like scanning tunneling microscopy and atomic force microscopy.
- The method developed is simple and scalable, making it compatible with cell culture and applicable to other cellular ligands that affect cell behavior based on their concentration.
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