The hexameric α-helical coiled-coil formed between the C-terminal and N-terminal heptad repeat (CHR and NHR) regions of class I viral fusion proteins plays an important role in mediating the fusion of the viral and cellular membranes and provides a clear starting point for molecular mimicry that drives viral fusion inhibitor design. Unfortunately, such peptide mimicry of the short α-helical region in the CHR of Middle East respiratory syndrome coronavirus (MERS-CoV) spike protein has been thwarted by the loss of the peptide's native α-helical conformation when taken out of the parent protein structure. Here, we describe that appropriate all-hydrocarbon stapling of the short helical portion-based peptide to reinforce its bioactive secondary structure remarkably improves antiviral potency. The resultant stapled peptide P21S10 could effectively inhibit infection by MERS-CoV pseudovirus and its spike protein-mediated cell-cell fusion; additionally, P21S10 exhibits improved pharmacokinetic properties than HR2P-M2, suggesting strong potential for development as an anti-MERS-CoV therapeutic.
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http://dx.doi.org/10.1021/acs.jmedchem.7b01732 | DOI Listing |
JASA Express Lett
January 2025
Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 92093,
This work demonstrates the feasibility of performing through-the-sensor (TTS) sub-bottom imaging using low-frequency ([100 Hz-1kHz]) self-noise generated by the propulsion of an autonomous underwater vehicle (AUV) acting as a source of opportunity. The self-noise was recorded by a short towed horizontal line array (11.4 m aperture) by the same AUV while it operated ∼35 m above the seabed along a range-dependent section at the New England shelf break.
View Article and Find Full Text PDFJMIR Form Res
January 2025
Department of Psychology, Lakehead University, Thunder Bay, ON, Canada.
Background: The minimal clinically important difference (MCID) is an important threshold to consider when evaluating the meaningfulness of improvement following an intervention. The JoyPop app is an evidence-based smartphone app designed to improve resilience and emotion regulation. Information is needed regarding the JoyPop app's MCID among culturally diverse youth.
View Article and Find Full Text PDFJMIR Res Protoc
January 2025
School of Human Nutrition, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Background: The 2019 Canada's Food Guide provides universal recommendations to individuals aged ≥2 years. However, the extent to which these recommendations are appropriate for older adults is unknown. Although ideal, conducting a large randomized controlled trial is unrealistic in the short term.
View Article and Find Full Text PDFJ Med Internet Res
January 2025
Centre for Research in Media and Communication, Faculty of Social Sciences and Humanities, Universiti Kebangsaan Malaysia, Selangor, Malaysia.
Background: Cardiovascular disease (CVD) is a major global health issue, with approximately 70% of cases linked to modifiable risk factors. Digital health solutions offer potential for CVD prevention; yet, their effectiveness in covering the full range of prevention strategies is uncertain.
Objective: This study aimed to synthesize current literature on digital solutions for CVD prevention, identify the key components of effective digital interventions, and highlight critical research gaps to inform the development of sustainable strategies for CVD prevention.
Br J Surg
December 2024
Department of Anaesthesiology, Nara Medical University, Nara, Japan.
Background: The WHO Disability Assessment Schedule (WHODAS) 2.0 is widely used for detecting postoperative functional disability. Its responsiveness for detecting disability has been evaluated at 1 year after surgery, with no long-term evaluation.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!