AI Article Synopsis
- This review contrasts traditional and miniaturized methods for preparing samples for transmission electron microscopy (TEM), with a focus on recent advancements in cryo-EM technology.
- It highlights how improvements in imaging technology and automation have enhanced cryo-EM's effectiveness, making it a powerful tool for structural analysis.
- The review identifies challenges in specimen preparation that have not progressed much in recent decades, and discusses new miniaturized methods that could lead to breakthroughs in areas like visual proteomics and time-resolved cryo-EM studies.
Article Abstract
This review compares and discusses conventional versus miniaturized specimen preparation methods for transmission electron microscopy (TEM). The progress brought by direct electron detector cameras, software developments and automation have transformed transmission cryo-electron microscopy (cryo-EM) and made it an invaluable high-resolution structural analysis tool. In contrast, EM specimen preparation has seen very little progress in the last decades and is now one of the main bottlenecks in cryo-EM. Here, we discuss the challenges faced by specimen preparation for single particle EM, highlight current developments, and show the opportunities resulting from the advanced miniaturized and microfluidic sample grid preparation methods described, such as visual proteomics and time-resolved cryo-EM studies.
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| http://dx.doi.org/10.1002/pmic.201700176 | DOI Listing |
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