AI Article Synopsis
- * Real-time PCR and multiplex PCR techniques were used to detect the ompA gene in ticks and to identify antibodies against SFG rickettsiae in the foxes' serum.
- * Results showed that 50.3% of serum samples were positive for SFG rickettsiae, with R. massiliae identified in 107 ticks, while no R. conorii was found, indicating that red foxes can host ticks carrying SFG rickettsia.
Article Abstract
To determine the prevalence of exposure to Rickettsia massiliae/Bar29 and Rickettsia conorii in wild red foxes, we collected blood samples and ticks from 135 foxes shot in different game reserve areas in Catalonia. To detect SFG rickettsia in Rhipicephalus sanguineus complex ticks collected from the foxes, we used real-time polymerase chain reaction (PCR) to screen for ompA gene and a tick-borne bacteria flow chip technique based on multiplex PCR. Serum samples were positive for antibodies against spotted fever group (SFG) rickettsiae in 68 (50.3%). Molecular techniques identified R. massiliae in 107 ticks, R. aeschlimannii in 3 ticks, and R. slovaca in one tick; no R. conorii was identified in any of the ticks analyzed. We conclude that red foxes can carry ticks with SFG rickettsia.
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| http://dx.doi.org/10.1016/j.ttbdis.2018.02.002 | DOI Listing |
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