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A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and in vivo. | LitMetric

A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and in vivo.

Biomater Sci

Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.

Published: February 2018

AI Article Synopsis

  • Sulfane sulfur is a unique form of ionized sulfur that has stronger chemical properties than hydrogen sulfide and may serve as a key signaling molecule in cells.
  • A new fluorescent probe called BD-diSH was developed to detect and image sulfane sulfur in living cells using near-infrared (NIR) fluorescence.
  • BD-diSH demonstrated high sensitivity and selectivity for sulfane sulfur and was successfully tested in both cell cultures and live mice, highlighting its potential for advancing research on sulfur compounds in biology.

Article Abstract

Sulfane sulfur refers to ionized sulfur that is reversibly attached to other sulfur atoms in the form of 6-valence electrons (S). Sulfane sulfur possesses stronger nucleophilicity and reducibility than hydrogen sulfide in a series of physiological reactions, which probably makes sulfane sulfur the actual signal molecule in cells. Herein, we designed and synthesized a near-infrared (NIR) fluorescent probe BD-diSH for sensitive detection and imaging of sulfane sulfur in living cells and in vivo. The probe BD-diSH is composed of two moieties: the fluorophore azo-BODIPY and the sulfane sulfur recognition unit, viz., 2-mercapto benzoate. BD-diSH displayed high sensitivity and selectivity towards sulfane sulfur. The mercapto group (-SH) of 2-mercapto benzoic acid can nucleophilically capture the sulfur atom of thiosulfoxide tautomers in sulfane sulfur to form -SSH. The group -SSH will immediately induce intramolecular cyclization reaction and release the azo-BODIPY fluorophore to emit NIR fluorescence. The probe BD-diSH was successfully applied to detect and image sulfane sulfur in the cytoplasm of the living cells. The results illustrated that the endogenous and exogenous sulfane sulfur level changed depending on different cell lines. BD-diSH was also capable of imaging the level changes of sulfane sulfur in mice. The above applications make our new probe a potential chemical tool for the study of physiological and pathological functions of sulfur sulfide in living cells and in vivo.

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Source
http://dx.doi.org/10.1039/c7bm00951hDOI Listing

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