(Papaya) latex: a new paradigm to combat against dengue and filariasis vectors and (Diptera: Culicidae).

This study manifests the larvicidal efficacy of latex extract and silver nanoparticles (CPAgNPs) synthesized using latex, against developing immature juveniles of and . Briefly, the latex was collected and fractioned with different solvents such as chloroform, methanol and aqueously. The obtained crude extracts were subjected to larvicidal activity in the dose-dependent method. After 24 h, the mortality rate was calculated and statistically analyzed. From the results, it was demonstrated that the chloroform extract displayed prominent activity in IInd and IIIrd instar larvae of and with better LC values followed by methanol and aqueous extract. Subsequently, we profiled the qualitative analysis of a chloroform extract through biochemical tests; Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. Moreover, we authenticated the major secondary metabolites and activated larvicidal compound present in the extract. Further, we synthesized CPAgNPs using aqueous latex extract and challenged with IInd and IIIrd instar larvae of and Noticeably, the synthesized nanoproducts were showed 100% mortality in a 24-h treatment with significant LC values. Hence, this study has opened up new vistas in the field of parasitological research to develop latex as a new stratagem in the insect vector management program.