Nowadays health benefits of bioactive food constituents, known as probiotic microorganisms, are a growing awareness. Cow's milk is a nutritious food containing probiotic bacteria. However, milk allergenicity is one of the most common food allergies. The milk protein, β-lactoglobulin (BLG), is in about 80% of all main cases of milk allergies for children and infants. With the aim of screening proteolytic strains of lactic acid bacteria to evaluate their potential for the reduction of allergenicity of the major bovine milk proteins, we isolated new proteolytic strains of cocci lactic acid bacteria from traditional Iranian dairy products. The proteases produced by these strains had strong proteolytic activity against BLG. Proteolysis of BLG, observed after sodium dodecyl sulfate-PAGE, was confirmed by the analysis of the peptide profiles by reversed-phase HPLC. The two isolates were submitted to 16S rDNA sequencing and identified as Lactcoccus lactis subsp. cremoris and Lactcoccus lactis subsp. hordniea. The competitive ELISA experiments confirmed that these isolates, with high proteolytic activity, reduce significantly the allergenicity of BLG. Accordingly, these isolates can reduce the immunoreactivity of bovine milk proteins, which can be helpful for the production of low-allergic dairy products.
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http://dx.doi.org/10.1016/j.ijbiomac.2018.02.044 | DOI Listing |
BMC Res Notes
December 2024
Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences and Ōmura Satoshi Memorial Institute, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.
Objective: We aimed to characterize integrative and conjugative elements (ICEs) in antimicrobial resistant Streptococcs uberis isolates from bovine milk in Chiba, Japan, based on whole-genome sequence (WGS) data.
Results: Of the 101 isolates, we found the 36 isolates harboring erm(B)-tet(O), showing resistance to macrolides-lincosamides-tetracyclines. The 22 isolates were randomly selected and subject to WGS determination.
Zhonghua Yu Fang Yi Xue Za Zhi
December 2024
Department of Anaphylaxis, Maternal and Child Health Hospital of Gansu Province, Lanzhou730050, China.
Cow's milk protein allergy is the most common food allergy in infants and young children, and its prevalence has been increasing in recent years. The use of appropriate diagnostic methods is essential for the management of patients. Oral food challenges are not yet widely used in clinical practice, and new and more effective methods are still being sought, including component resolved diagnosis.
View Article and Find Full Text PDFJ Dairy Sci
December 2024
Grupo de Investigación en Procesos Agroindustriales, Faculty of Engineering, Universidad de La Sabana, Campus Universitario del Puente del Común, Km 7 Autopista Norte de Bogotá, Chía, Cundinamarca, Colombia. Electronic address:
Refractance window drying is an emerging technology that allows the development of new dried foods with an acceptable shelf life from products widely consumed in the world with high nutritional content and health benefits, such as Dairy products. The present study aimed to determine the effect of temperature and product thickness during Refractance window drying in a laboratory scale dryer on the physicochemical properties of whole bovine milk and commercial flavored yogurt, using an optimal design model. The drying temperature range was between 40°C and 80°C, while the evaluated thickness ranged from 1 to 3 mm.
View Article and Find Full Text PDFJ Dairy Sci
December 2024
Department of Animal Science, University of California, Davis, CA 95616.
Grape pomace (GP) is a byproduct of the viticulture industry and shows promise for feeding dairy cattle as well as reducing enteric methane (CH) emissions. This study investigates the potential of using fresh GP in dairy cow feeding and its effects on CH emissions. Multiparous Holstein dairy cows (n = 24, 205 ± 39 DIM) were housed in free stall barns and provided rations consisting of alfalfa hay, wheat hay, almond hulls, cottonseed, and grain mix.
View Article and Find Full Text PDFJ Nutr
December 2024
National Center for International Research on Animal Gut Nutrition, Jingsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Nanjing Agricultural University, Nanjing, 210095 China; College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address:
Background: Rumen methane emissions (RME) significantly contribute to global greenhouse gas emissions, underscoring the essentials to identify effective inhibitors for RME mitigation. Despite various inhibitors shown potential in reducing RME by modulating rumen microbes, their impacts include considerable variations and inconsistency.
Objective: We aimed to quantitively assess the impacts of various methane inhibitors on RME, rumen microbial abundance and fermentation in ruminants.
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