AI Article Synopsis
- The gonad serves as a valuable system for studying how cells decide their fates, especially concerning somatic progenitor cells and the transcription processes involved in cell differentiation.
- Through time-series single-cell RNA sequencing of XY mouse gonads, researchers identified a distinct group of somatic progenitor cells that exist before sex determination.
- The study reveals that some of these progenitors evolve into Sertoli cells with a dynamic genetic program, while others change transcriptionally to restrict their capabilities toward becoming fetal Leydig cells, highlighting a unique multipotent progenitor population in gonadal development.
Article Abstract
The gonad is a unique biological system for studying cell-fate decisions. However, major questions remain regarding the identity of somatic progenitor cells and the transcriptional events driving cell differentiation. Using time-series single-cell RNA sequencing on XY mouse gonads during sex determination, we identified a single population of somatic progenitor cells prior to sex determination. A subset of these progenitors differentiates into Sertoli cells, a process characterized by a highly dynamic genetic program consisting of sequential waves of gene expression. Another subset of multipotent cells maintains their progenitor state but undergoes significant transcriptional changes restricting their competence toward a steroidogenic fate required for the differentiation of fetal Leydig cells. Our findings confirm the presence of a unique multipotent progenitor population in the gonadal primordium that gives rise to both supporting and interstitial lineages. These also provide the most granular analysis of the transcriptional events occurring during testicular cell-fate commitment.
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| http://dx.doi.org/10.1016/j.celrep.2018.01.043 | DOI Listing |
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