Design of corneal endothelium substitute is important for replacement of cadaveric cornea tissue. Our previous study has shown the suitability of silk fibroin (SF) as a biomaterial for cornea scaffold. In this study, we used β-Carotene (β-C) to enhance the regeneration of corneal endothelial cells (CEnCs) and maintain CEnC specific function. The fabricated film scaffolds showed desired transparency and hydrophilic properties which are crucial factors for vision recovery. The cell viability, phenotype and gene expression was examined using MTT assay, immunofluorescence and reverse transcription polymerase chain reactions. Compared with pristine SF scaffold, proper amount of β-C incorporated with SF scaffolds showed higher initial cell attachment, cell viability and mRNA expression. The results indicate that the fabricated SF film scaffold incorporated with small amount of β-C might be the suitable alternative corneal endothelium substitute for transplantation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.colsurfb.2017.11.052 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mechanical Characterization of Amniotic-Based Scaffolds Containing Silk Fibroin and Sodium Alginate Nanofibers.
J Biomed Mater Res B Appl Biomater
February 2025
School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, Tehran, Iran.
Due to its availability and biocompatibility, the human amniotic membrane (hAM) is being investigated by a large number of researchers with the goal of gaining a better understanding of the materials' mechanical behavior and structural integrity and optimizing them for various Tissue Engineering applications. In this research, biopolymers sodium alginate (SA) and silk fibroin (SF) were electrospun onto a decellularized hAM, resulting in two types of hybrid scaffolds: hAM/SF and hAM/SF/SA. The mechanical characteristics of these nanofibers were then analyzed to guide scaffold optimization for applications using these materials.
View Article and Find Full Text PDFRevealing the promising era of silk-based nanotherapeutics: a ray of hope for chronic wound healing treatment.
Naunyn Schmiedebergs Arch Pharmacol
January 2025
Department of Pharmaceutics, School of Pharmaceutical Sciences, Delhi Pharmaceutical Sciences and Research University, Pushp Vihar, New Delhi, 110017, India.
Chronic wounds significantly contribute to disability and affect the mortality rate in diabetic patients. In addition, pressure ulcers, diabetic foot ulcers, arterial ulcers, and venous ulcers pose a significant health burden due to their associated morbidity and death. The complex healing process, environmental factors, and genetic factors have been identified as the rate-limiting stages of chronic wound healing.
View Article and Find Full Text PDFNovel Silk Fibroin Based Bilayer Scaffolds for Bioabsorbable Internal Biliary Stenting.
J Biomed Mater Res B Appl Biomater
February 2025
Fibrothelium GmbH, Aachen, Germany.
Biliary duct reconstruction is one of the most challenging parts of liver transplantation and accounts for 40%-60% of complications. While current stent-based devices on the market show promising results in reducing complications, they are manufactured from permanent synthetic materials and require a second reintervention for their removal. This exposes the patients to other potential complications and increases healthcare costs.
View Article and Find Full Text PDFMussel-Like Silk Fibroin Hydrogel With Skin Compliance Soft Electrode for Wearable Devices.
Macromol Rapid Commun
January 2025
Department of Physics, College of Physical Science and Technology, Research Institution for Biomimetics and Soft Matter, Xiamen University, Xiamen, Fujian, 361005, China.
Flexible wearable electronic devices, capable of real-time physiological monitoring for personalized health management, are increasingly recognized for their convenience, comfort, and customization potential. Despite advancements, challenges persist for soft electrodes due to the skin's complex surface, biocompatibility demands, and modulus mismatch. In response, a mussel-inspired polydopamine-nanoclay-silk fibroin hydrogel (DA-C-SFH) is introduced, synthesized via a two-step process.
View Article and Find Full Text PDFPeripheral nerve regeneration using a bioresorbable silk fibroin-based artificial nerve conduit fabricated via a novel freeze-thaw process.
Sci Rep
January 2025
Department of Orthopaedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-Ku, Tokyo, 160-8582, Japan.
While silk fibroin (SF) obtained from silkworm cocoons is expected to become a next-generation natural polymer, a fabrication method for SF-based artificial nerve conduits (SFCs) has not yet been established. Here, we report a bioresorbable SFC, fabricated using a novel freeze-thaw process, which ensures biosafety by avoiding any harmful chemical additives. The SFC demonstrated favorable biocompatibility (high hydrophilicity and porosity with a water content of > 90%), structural stability (stiffness, toughness, and elasticity), and biodegradability, making it an ideal candidate for nerve regeneration.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!