The resolution of racemates often requires difficult and time consuming purification procedures. McAb technology allows the production of specific antibodies in quantities suitable for the preparation of matrices for large scale affinity purification. Here we report the rapid separation of abscisic acid (ABA) enantiomers by affinity chromatography using McAb. This method appears to be far superior to previously published separations based on crystallization, chromatography, and affinity purification with conventional antisera. The approach here described will be particularly attractive in a wide variety of similar situations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0003-2697(86)90230-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The ASPARAGINE-RICH PROTEIN-LYST-INTERACTING PROTEIN5 complex regulates non-canonical AUTOPHAGY8 degradation in Arabidopsis.
Plant Physiol
January 2025
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin, 300071, China.
The endocytic and autophagic pathways play important roles in abiotic stress responses and maintaining cellular homeostasis in plants. Asparagine Rich Proteins (NRPs) are plant-specific stress-responsive proteins that are involved in many abiotic stress-related signaling pathways. We previously demonstrated that NRP promotes PIN FORMED 2 (PIN2) vacuolar degradation to maintain PIN2 homeostasis under abscisic acid (ABA) treatment in Arabidopsis (Arabidopsis thaliana).
View Article and Find Full Text PDFGenome-wide identification and characterization of cation-proton antiporter (CPA) gene family in rice (Oryza sativa L.) and their expression profiles in response to phytohormones.
PLoS One
January 2025
Laboratory of Functional Genomics and Proteomics, Department of Genetic Engineering and Biotechnology, Faculty of Biological Science and Technology, Jashore University of Science and Technology, Jashore, Bangladesh.
The cation-proton antiporter (CPA) superfamily plays pivotal roles in regulating cellular ion and pH homeostasis in plants. To date, the regulatory functions of CPA family members in rice (Oryza sativa L.) have not been elucidated.
View Article and Find Full Text PDFA Chromosome-Level Genome Sequence Reveals Regulation of Salt Stress Response in Mesembryanthemum crystallinum.
Physiol Plant
January 2025
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan.
Salt stress disturbs plant growth and photosynthesis due to its toxicity. The ice plant Mesembryanthemum crystallinum is a highly salt-tolerant facultative crassulacean acid metabolism (CAM) plant. However, the genetic basis of the salt tolerance mechanisms in ice plants remains unclear.
View Article and Find Full Text PDFPlant Coping with Cold Stress: Molecular and Physiological Adaptive Mechanisms with Future Perspectives.
Cells
January 2025
Henan Collaborative Innovation Centre of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, China.
Cold stress strongly hinders plant growth and development. However, the molecular and physiological adaptive mechanisms of cold stress tolerance in plants are not well understood. Plants adopt several morpho-physiological changes to withstand cold stress.
View Article and Find Full Text PDFA chromosome-scale genome assembly of the pioneer plant Stylosanthes angustifolia: insights into genome evolution and drought adaptation.
Gigascience
January 2025
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, China.
Background: Drought is a major limiting factor for plant survival and crop productivity. Stylosanthes angustifolia, a pioneer plant, exhibits remarkable drought tolerance, yet the molecular mechanisms driving its drought resistance remain largely unexplored.
Results: We present a chromosome-scale reference genome of S.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!