AI Article Synopsis
- - This study focuses on creating and optimizing insulin nanoparticles using a specific statistical design (Box-Behnken) and a fabrication method (ionic gelation) involving a thiolated chitosan conjugate.
- - The optimized nanoparticles, averaging 148nm in size, demonstrated effective drug loading into buccal films, with notable drug release properties assessed through in vitro studies, including FTIR analysis.
- - Cell viability tests using an MTT assay confirmed that these nanoparticles and films are safe for cells and show promise as oral delivery systems for insulin.
Article Abstract
This study aims at the mathematical optimization by Box-Behnken statistical design, fabrication by ionic gelation technique and in vitro characterization of insulin nanoparticles containing thiolated N- dimethyl ethyl chitosan (DMEC-Cys) conjugate. Then Optimized insulin nanoparticles were loaded into the buccal film, and in-vitro drug release from films was investigated, and diffusion coefficient was predicted. The optimized nanoparticles were shown to have mean particle size diameter of 148nm, zeta potential of 15.5mV, PdI of 0.26 and AE of 97.56%. Cell viability after incubation with optimized nanoparticles and films were assessed using an MTT biochemical assay. In vitro release study, FTIR and cytotoxicity also indicated that nanoparticles made of this thiolated polymer are suitable candidates for oral insulin delivery.
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| http://dx.doi.org/10.1016/j.ijbiomac.2018.01.215 | DOI Listing |
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