Development of new and specific insect pest management methods is critical for overcoming pesticide resistance and collateral off-target killings. Gene silencing by feeding dsRNA to insects shows promise in this area. Here we described the use of a peptide nano-material, branched amphiphilic peptide capsules (BAPCs), that facilitates cellular uptake of dsRNA by insects through feeding. The insect diets included dsRNA with and without complexation with BAPCs. The selected insect species come from two different orders with different feeding mechanisms: Tribolium castaneum and Acyrthosiphon pisum. The gene transcripts tested (BiP and Armet) are part of the unfolded protein response (UPR) and suppressing their translation resulted in lethality. For Acyrthosiphon pisum, ingestion of BiP-dsRNA associated with BAPCs led to the premature death of the aphids (t=4-5days) compared to ingestion of the same amounts of free BiP-dsRNA (t=11-12days). Tribolium castaneum was effectively killed using a combination of BiP-dsRNA and Armet-dsRNA complexed with BAPCs; most dying as larvae or during eclosion (~75%). Feeding dsRNA alone resulted in fewer deaths (~30%). The results show that complexation of dsRNA with BAPCs enhanced the oral delivery of dsRNA over dsRNA alone.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984438 | PMC |
| http://dx.doi.org/10.1016/j.jconrel.2018.01.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Inferring Tripartite Associations of Vector-Borne Plant Pathogens Using a Next-Generation Sequencing Approach.
Pathogens
January 2025
Illinois Natural History Survey, Prairie Research Institute, University of Illinois at Urbana-Champaign, Champaign, IL 61820, USA.
Phytoplasmas are a group of plant-pathogenic, cell-wall-less bacteria vectored primarily by leafhoppers (Hemiptera Cicadellidae), one of the most diverse families of insects. Despite the importance of documenting associations between phytoplasmas, their insect vectors, and plant hosts to prevent disease outbreaks, such knowledge is currently highly incomplete and largely neglects the diversity of the system in natural areas. Here, we used anchored hybrid enrichment (AHE) to recover the DNA of five plant genes (, , , , and ) in 58 phloem-feeding leafhoppers from around the world that had previously tested positive for phytoplasma infection.
View Article and Find Full Text PDFPreliminary Research on the Health-Promoting Value of Honeydew Honey Enriched with Bee Bread.
Molecules
January 2025
The National Institute of Horticultural Research, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland.
Since the imbalance between free radicals and antioxidants in the body plays a significant role in the physiology of common, often dangerous diseases, an emphasis is placed on enriching the daily diet with compounds characterized by antioxidant activity. Good sources of natural antioxidants are bee products such as honey, bee pollen, bee bread and propolis, and the best path for introducing the latter products into the diet is mixing them with honey. However, the characteristics of bee product mixtures are not yet fully understood.
View Article and Find Full Text PDFFunctional Responses of the Warehouse Pirate Bug (Reuter) (Hemiptera: Anthocoridae) on a Diet of (Pearman) (Psocodea: Liposcelididae).
Insects
January 2025
Department of Statistics, Oklahoma State University, 301 Mathematics, Statistics and Computer Sciences, Stillwater, OK 74078, USA.
Psocids are difficult to manage using grain protectants and phosphine hence research on non-chemical control methods. This study evaluated the effectiveness of (Reuter) (Hemiptera: Anthocoridae) at managing (Pearman) (Psocodea: Liposcelididae). The functional responses of adult♀ and nymphs of on a diet of nymphs, adult♂, and adult♀ of were determined under laboratory conditions at 28 ± 1 °C, 63 ± 5% RH, and a 0:24 (L:D) photoperiod.
View Article and Find Full Text PDFAn Overview of the Nutritional Requirements of Honey Bees ( Linnaeus, 1758).
Insects
January 2025
Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, Slovenia.
Honey bees are known for their wide global distribution, their ease of handling, and their economic and ecological value. However, they are often exposed to a wide variety of stress factors. Therefore, it is essential for beekeepers to maintain healthy bee colonies.
View Article and Find Full Text PDFin the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the Larvae.
Insects
January 2025
Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
This study assessed the bioconversion efficiency of larvae (BSFL) fed on food waste stored under different conditions, focusing on the nutritional and microbial quality of the resulting larval biomass. Food waste was prepared as a fresh diet (FD) or naturally contaminated and stored at 20-22 °C (OS-T, opened storage-tempered) or under refrigeration, at 5-8 °C (CS-C, closed storage-cooled). Refrigerated, closed storage (CS-C) led to the highest rates of waste reduction (91.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!