AI Article Synopsis
Article Abstract
The HPLC-MS/MS method was developed to profile the dynamics of abscisic acid (ABA) and ABA-glucose ester (ABA-GE) after cloning glycosyltransferase enzyme family gene into . By constructing over-expression lines (OE) and down-expression lines (DN), we acquired mutant strains to analyze the function of . The multiple-reaction monitoring (MRM) was used for quantitative determination in negative mode. The transition was / 263.1→153.0 for ABA ([M-H]), / 425.1→263.0 for ABA-GE ([M-H]), and / 321.0→152.0 for chloramphenicol. The linear range was 0.8684-217.1 ng/mL for ABA and 0.3920-196.0 ng/mL for ABA-GE. The accuracy was 88.0-109.0% for ABA and 86.6-113.0% for ABA-GE; the inter-day and intra-day precisions were less than 5.4% for ABA and 8.9% for ABA-GE, respectively. This method is simple and sensitive enough for determination of ABA and ABA-GE in leaves. All the evidence confirmed the speculation that can mediate abscisic acid homeostasis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761124 | PMC |
| http://dx.doi.org/10.1016/j.jpha.2014.01.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tandem mass tag-based quantitative proteomics and targeted hormone analysis reveal the response to insect herbivory stress in Ginseng (Panax ginseng, L.).
PLoS One
January 2025
Wuzhou University, College of Food and Pharmaceutical Engineering, Guangxi, P. R. China.
Ginsenosides are the most important secondary metabolites of ginseng. Ginseng has developed certain insect resistance properties during the course of evolutionary environmental adaptation. However, the mechanism underlying the insect resistance of ginseng is poorly understood.
View Article and Find Full Text PDFThe Interaction Between ABA and Sugar Signalling Regulates Stomatal Production in Systemic Leaves by Controlling Sucrose Transport.
Plant Cell Environ
January 2025
College of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui, China.
A key feature of stress responses [closely relative to the phytohormone abscisic acid (ABA)] and associated acclimation in plants is the dynamic adjustments and related optimisation of carbohydrate content between sink and source organs. The production of stomata, which consist of a pore between two adjacent guard cells, are central to plant adaptation to changing environment conditions. In this context, ABA is a core modulator of environmentally determined stomatal development.
View Article and Find Full Text PDFApoplastic pH is a chemical switch for extracellular HO signaling in abscisic acid-mediated inhibition of cotyledon greening.
New Phytol
January 2025
State Key Laboratory of Plant Environmental Resilience, Zhejiang University, Hangzhou, 310058, China.
The apoplastic pH (pH) in plants is susceptible to environmental stimuli. However, the biological implications of pH variation have remained largely unknown. The universal stress phytohormone abscisic acid (ABA) as well as the major environmental stimuli drought and salinity were selected as representative cases to investigate how changes in pH relate to plant behaviors in Arabidopsis.
View Article and Find Full Text PDFGene expression and mucilage adaptations to salinity in germination of extreme halophyte Schrenkiella parvula seeds.
Plant Physiol Biochem
January 2025
Department of Biology, Faculty of Science, Ege University, Izmir, Türkiye. Electronic address:
Salinization is a significant global issue causes irreversible damage to plants by reducing osmotic potential, inhibiting seed germination, and impeding water uptake. Seed germination, a crucial step towards the seedling stage is regulated by several hormones and genes, with the balance between abscisic acid and gibberellin being the key mechanism that either promotes or inhibits this process. Additionally, mucilage, a gelatinous substance, is known to provide protection against drought, herbivory, soil adhesion, and seed sinking.
View Article and Find Full Text PDFGenome-wide analysis of the SPL family in Zanthoxylum armatum and ZaSPL21 promotes flowering and improves salt tolerance in transgenic Nicotiana benthamiana.
Plant Mol Biol
January 2025
Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, China.
Z. armatum is an economically valued crop known for its rich aroma and medicinal properties. This study identified 45 members of the SQUAMOSA-PROMOTER BINDING PROTEIN LIKE (SPL) gene family in the genome of Z.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!