An efficient method for the isolation and purification of 12,13-dihydroxyeuparin from by high speed counter-current chromatography (HSCCC) was established in this paper. The ether extracts of were purified by HSCCC with a solvent system of hexyl hydride-ethyl acetate-methanol-water (1:2:1:2, v/v/v/v). The upper phase was used as the stationary phase and the lower phase as the mobile phase. About 8.4 mg of 12,13-dihydroxyeuparin was obtained from 200 mg of ether extracts from in one-step HSCCC separation, with the purity of 96.71%, as determined by HPLC. After methanol-water recrystallization, the purity of 12,13-dihydroxyeuparin reached 99.83%. Such a simple and effective method was fairly useful to prepare pure compound as reference substances for related study on
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| http://dx.doi.org/10.1016/j.jpha.2012.03.004 | DOI Listing |
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