GG Affects Microbiota and Suppresses Autophagy in the Intestines of Pigs Challenged with Infantis.

serovar Infantis (. Infantis) is a common source of foodborne gastroenteritis worldwide. Here, GG (LGG) was administrated to weaned piglets for 1 week before . Infantis challenge. . Infantis caused decreased ileal mucosal microbiota diversity, a dramatic bloom, and decreased abundance of , and . The beneficial effect of LGG correlated with the moderate expansion of , and several members of the phyla , and . . Infantis translocation to the liver was decreased in the LGG-pretreated piglets. An model of LGG and . Infantis co-incubation (involving the porcine intestinal epithelial cell line IPEC-J2) was established, and nalidixic acid was used to kill the extracellular . Infantis. LGG suppressed the initial . Infantis invasion in the IPEC-J2 cells and deceased the rate of cell death. LGG inhibited . Infantis-induced autophagy and promoted epidermal growth factor receptor (EGFR) and Akt phosphorylation in both the ileum and IPEC-J2 cells. Our findings suggest that LGG inhibited . Infantis-induced autophagy by promoting EGFR-mediated activation of the negative mediator Akt, which, in turn, suppressed intestinal epithelial cell death and thus restricted systemic . Infantis infection. LGG can restore the gut microbiota balance and preserve the autophagy-related intestinal epithelial barrier, thereby controlling infections.