Molecular characterization and complete genome of a novel nepovirus from red clover.

Arch Virol

Department of Plant Virology, Biology Centre, Institute of Plant Molecular Biology, The Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic.

Published: May 2018

During high throughput sequencing (HTS) of leaves from a symptomatic red clover plant, a new RNA virus, tentatively named red clover nepovirus A (RCNVA), was discovered. The complete genomic sequence was determined and characterized. Particularly noteworthy was that RCNVA shares high sequence identities in RNA1 with a group of phylogenetically related nepoviruses while homologies in the RNA2 segments are markedly lower. Based on the genomic organization and phylogenetic attributes, RCNVA should be classified as a novel virus of the genus Nepovirus (subfamily Comovirinae, family Secoviridae, order Picornavirales).

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http://dx.doi.org/10.1007/s00705-018-3742-9DOI Listing

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