AI Article Synopsis
- Monitoring enzymatic activities at cell surfaces is tough due to limitations in FRET-based biosensors, which struggle with transport and membrane integration.
- A new hybrid biosensor was developed using a monobody variant (PEbody) that binds to a specific dye, enabling real-time visualization of intercellular junction dynamics.
- This biosensor demonstrated varying levels of MT1-MMP activities at different types of cell-cell contacts, highlighting the potential of directed evolution and design techniques for better monitoring molecular interactions in live cells.
Article Abstract
Monitoring enzymatic activities at the cell surface is challenging due to the poor efficiency of transport and membrane integration of fluorescence resonance energy transfer (FRET)-based biosensors. Therefore, we developed a hybrid biosensor with separate donor and acceptor that assemble in situ. The directed evolution and sequence-function analysis technologies were integrated to engineer a monobody variant (PEbody) that binds to R-phycoerythrin (R-PE) dye. PEbody was used for visualizing the dynamic formation/separation of intercellular junctions. We further fused PEbody with the enhanced CFP and an enzyme-specific peptide at the extracellular surface to create a hybrid FRET biosensor upon R-PE capture for monitoring membrane-type-1 matrix metalloproteinase (MT1-MMP) activities. This biosensor revealed asymmetric distribution of MT1-MMP activities, which were high and low at loose and stable cell-cell contacts, respectively. Therefore, directed evolution and rational design are promising tools to engineer molecular binders and hybrid FRET biosensors for monitoring molecular regulations at the surface of living cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910193 | PMC |
| http://dx.doi.org/10.1016/j.chembiol.2018.01.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Laser Synthesis of Platinum Single-Atom Catalysts for Hydrogen Evolution Reaction.
Nanomaterials (Basel)
January 2025
Department of Materials, School of Natural Science, The University of Manchester, Oxford Road, Manchester M13 9PL, UK.
Platinum (Pt)-based heterogeneous catalysts show excellent performance for the electrocatalytic hydrogen evolution reaction (HER); however, the high cost and earth paucity of Pt means that efforts are being directed to reducing Pt usage, whilst maximizing catalytic efficiency. In this work, a two-step laser annealing process was employed to synthesize Pt single-atom catalysts (SACs) on a MOF-derived carbon substrate. The laser irradiation of a metal-organic framework (MOF) film (ZIF67@ZIF8 composite) by rapid scanning of a ns pulsed infrared (IR; 1064 nm) laser across the freeze-dried MOF resulted in a metal-loaded graphitized film.
View Article and Find Full Text PDFGraphitic Carbon Nitride for Photocatalytic Hydrogen Production from Water Splitting: Nano-Morphological Control and Electronic Band Tailoring.
Nanomaterials (Basel)
December 2024
State Key Laboratory of Solidifcation Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an 710072, China.
Semiconductor polymeric graphitic carbon nitride (g-CN) photocatalysts have garnered significant and rapidly increasing interest in the realm of visible light-driven hydrogen evolution reactions. This interest stems from their straightforward synthesis, ease of functionalization, appealing electronic band structure, high physicochemical and thermal stability, and robust photocatalytic activity. This review starts with the basic principle of photocatalysis and the development history, synthetic strategy, and structural properties of g-CN materials, followed by the rational design and engineering of g-CN from the perspectives of nano-morphological control and electronic band tailoring.
View Article and Find Full Text PDFTransition metal phosphide-based oxygen electrocatalysts for aqueous zinc-air batteries.
Chem Commun (Camb)
January 2025
Functional Materials and Electrochemistry Lab, Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, West Bengal, India.
Electrically rechargeable zinc-air batteries (ZABs) are emerging as promising energy storage devices in the post-lithium era, leveraging the oxygen reduction reaction (ORR) and the oxygen evolution reaction (OER) at the air cathodes. Efficient bifunctional oxygen electrocatalysts, capable of catalyzing both the ORR and OER, are essential for the operation of rechargeable ZABs. Traditional Pt- and RuO/IrO-based catalysts are not ideal, as they lack sufficient bifunctional ORR and OER activity, exhibit limited long-term durability, require high overpotentials and are expensive.
View Article and Find Full Text PDFSpeciation studies in the genomic era.
Yi Chuan
January 2025
State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou 730000, China.
Since Darwin's era, speciation has been one of the most central issues in evolutionary biology studies. Understanding the processes of species origin is crucial in deepening our understanding of the formation of species biodiversity, which is essential for their protections. However, speciation research has been challenging due to the rather complex evolutionary histories of many extant species.
View Article and Find Full Text PDFA digital program for daily life management with endometriosis: Pilot study on symptoms and quality of life among participants.
JMIR Form Res
January 2025
Lyv Healthcare, 6 rue Edouard Nignon, Nantes, FR.
Background: After suffering for an average of 7 years before diagnosis, endometriosis patients are usually left with more questions than answers about managing their symptoms in the absence of a cure. To help women with endometriosis after their diagnosis, we developed an online support program combining user research, evidence-based medicine, and clinical expertise. Structured around CBT and the quality-of-life metrics from the EHP score, the program is designed to guide participants over a 3-month and is available in France.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!