Background And Aims: We aim to identify significant transcriptome alterations of vascular smooth muscle cells (VSMCs) in the aortic wall of myocardial infarction (MI) patients. Providing a robust transcriptomic signature, we aim to highlight the most likely aberrant pathway(s) in MI VSMCs.
Methods And Results: Laser-captured microdissection (LCM) was used to obtain VSMCs from aortic wall tissues harvested during coronary artery bypass surgery. Microarray gene analysis was applied to analyse VSMCs from 17 MI and 19 non-MI patients. Prediction Analysis of Microarray (PAM) identified 370 genes that significantly discriminated MI and non-MI samples and were enriched in genes responsible for muscle development, differentiation and phenotype regulation. Incorporation of gene ontology (GO) led to the identification of a 21-gene VSMCs-associated classifier that discriminated between MI and non-MI patients with 92% accuracy. The mass spectrometry-based iTRAQ analysis of the MI and non-MI samples revealed 94 proteins significantly differentiating these tissues. Ingenuity Pathway Analysis (IPA) of 370 genes revealed top pathways associated with hypoxia signaling in the cardiovascular system. Enrichment analysis of these proteins suggested an activation of the superoxide radical degradation pathway. An integrated transcriptome-proteome pathway analysis revealed that superoxide radical degradation pathway remained the most implicated pathway. The intersection of the top candidate molecules from the transcriptome and proteome highlighted superoxide dismutase (SOD1) overexpression.
Conclusions: We provided a novel 21-gene VSMCs-associated MI classifier in reference to significant VSMCs transcriptome alterations that, in combination with proteomics data, suggests the activation of superoxide radical degradation pathway in VSMCs of MI patients.
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http://dx.doi.org/10.1016/j.atherosclerosis.2018.01.024 | DOI Listing |
Vet Med Sci
January 2025
College of Agriculture and Animal Husbandry, Qinghai University, Xining, People's Republic of China.
With global protein prices on the rise, lowering protein levels in animal feed, together with balancing diet composition and reducing nitrogen emissions, can both reduce the environmental impact of agriculture and save on feed costs. However, the formulation of an ideal amino acid (AA) composition is crucial for better protein utilization by livestock. This study aimed to investigate the effects of different lysine to methionine ratios on the antioxidant capacity and immune function of the rumen in Tibetan sheep.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
December 2024
Center of Excellence for Innovation in Chemistry, Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, 90110, Songkhla, Thailand.
CrO/g-CN photocatalyst was successfully synthesized via the one-pot thermal polycondensation method by mixing different ratios of CrCl.HO and thiourea. Thiourea was used as the precursor for building g-CN.
View Article and Find Full Text PDFSci Bull (Beijing)
December 2024
School of Chemistry and Chemical Engineering/Institute of Clean Energy and Materials/Guangzhou Key Laboratory for Clean Energy and Materials/Huangpu Hydrogen Innovation Center, Guangzhou University, Guangzhou 510006, China. Electronic address:
Photocatalytic hydrogen peroxide (HO) generation is largely subject to the sluggish conversion kinetics of the superoxide radical (O) intermediate, which has relatively low reactivity and requires high energy. Here, we present a lattice-strain strategy to accelerate the conversion of O to highly active singlet oxygen(O) by optimizing the distance between two adjacent active sites, thereby stimulating HO generation via low-barrier oxygen-oxygen coupling. As the initial demonstration, the defect-induced strain in ZnInS nanosheet optimizes the distance of two adjacent Zn sites from 3.
View Article and Find Full Text PDFEnviron Res
December 2024
School of Advanced Chemical Sciences, Shoolini University, Solan Himachal Pradesh-173212, India. Electronic address:
The study presents the fabrication and superior photoactivity of a ternary g-CN/FeVO/AgBr heterojunction nanocomposite, synthesized via a chemical precipitation method for effective degradation of tetracycline (TC) and Victoria Blue (VB) dye under light illumination. The morphology and the crystal size of the synthesized nanocomposite were characterized by using FESEM and XRD and the calculated grain size (100.39 nm) is larger than the crystal size (48.
View Article and Find Full Text PDFBMC Chem
December 2024
Department of Chemical Sciences, Faculty of Natural Sciences, Redeemer's University, PMB 230, Ede, Nigeria.
This Study focuses on the preparation of sustainable and efficient Chitosan catalyst for the removal of three organic pollutants, 17β-Estradiol (E2), 17α-ethynyl estradiol (EE2) and triclosan (TCS) from water. The prepared nanocomposites were characterized by different techniques which confirmed the presence of the key components Chitosan, Carica Papaya seed and Kaolinite. The optical characterization proved the nanocomposite is photoactive with a band gap of 1.
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