Nitric oxide (NO) is gaining increasing attention as a central molecule with diverse signaling functions. It has been shown that NO acts as a negative regulator of leaf senescence. In this chapter, we describe a highly selective method, electron paramagnetic resonance ([EPR], also known as electron spin resonance [ESR]), for NO determination in leaf senescence. An iron complex of ferrous and mononitrosyl dithiocarbamate (Fe(DETC)) is used as a chelating agent for NO. Using ethyl acetate as extracting solvent, the NOFe(DETC) complex is extracted and determined by EPR spectrometer.
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