Extracellular RNAs are emerging as novel biomarkers and mediators of intercellular communication. Various methods to isolate RNA from biofluids and cell culture supernatants have been previously used by investigators. Here, we describe several standardized protocols for the isolation of RNAs from cell culture supernatants that utilize commercially available kits and reagents.
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NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice.
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View Article and Find Full Text PDFBioengineering Approaches for Male Infertility: From Microenvironmental Regeneration to in vitro Fertilization.
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METU MEMS Center, Ankara, Turkey.
Male factor accounts for 30-50% of infertility cases and may occur due to congenital anomalies or acquired disorders. In such infertility cases where a limited number of mature sperm is produced, a solution is offered to patients with ART applications; however, these methods are inadequate in patients with germ cell aplasia due to damaged microenvironment. Since monolayer cell culture and static culture conditions do not provide the physical conditions of the 3D microenvironment, they have a limited effect on ensuring the execution of in vitro spermatogenesis properly.
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Department of Bioprocess Engineering, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. Electronic address:
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