A red-emitting fluorescent probe for hydrogen sulfide in living cells with a large Stokes shift.

A red-emitting fluorescent probe was developed for the sensitive and selective detection of HS. Upon treatment with HS, this probe exhibited a remarkable fluorescence enhancement (10 fold) with a large Stokes shift (125 nm). The detection limit of this probe was as low as 5.7 nM based on S/N = 3. The application of this probe in the detection of intracellular HS in living cells is successfully demonstrated.