AI Article Synopsis
- The TMJ can adapt to external forces, which influence the position of its components and the structure of the mandibular condylar cartilage (MCC).
- The manuscript outlines techniques for evaluating these changes, including a method for applying compressive static loading in mice.
- It describes a simple morphometric analysis using Digimizer software and methods for quantifying cellular changes in the MCC, making these techniques applicable beyond just studying mice.
Article Abstract
The temporomandibular joint (TMJ) has the capacity to adapt to external stimuli, and loading changes can affect the position of condyles, as well as the structural and cellular components of the mandibular condylar cartilage (MCC). This manuscript describes methods for analyzing these changes and a method for altering the loading of the TMJ in mice (i.e., compressive static TMJ loading). The structural evaluation illustrated here is a simple morphometric approach that uses the Digimizer software and is performed in radiographs of small bones. In addition, the analysis of cellular changes leading to alterations in collagen expression, bone remodeling, cell division, and proteoglycan distribution in the MCC is described. The quantification of these changes in histological sections - by counting the positive fluorescent pixels using image software and measuring the distance mapping and stained area with Digimizer - is also demonstrated. The methods shown here are not limited to the murine TMJ, but could be used on additional bones of small experimental animals and in other regions of endochondral ossification.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908544 | PMC |
| http://dx.doi.org/10.3791/55998 | DOI Listing |
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