AI Article Synopsis
- The study used next-generation sequencing (NGS) to investigate the transmission and evolution of HIV-1 in a heterosexual couple over 54 months.
- Both partners were found to be infected with the same subtype of HIV-1, CRF65_cpx, and showed a gradual increase in gene diversity and separation in their HIV-1 quasispecies over time.
- The findings suggest that NGS is a more reliable method for HIV-1 tracking, which can enhance the development of targeted public health interventions.
Article Abstract
Next-generation sequencing (NGS) has been successfully used to trace HIV-1 infection. In this study, we investigated the transmission and evolution of HIV-1 quasispecies in a couple infected through heterosexual behavior. A heterosexual couple in which both partners were infected with HIV-1 was followed up for 54 months. Blood samples including whole-blood and plasma samples, were collected at various time points. After HIV-1 subtyping, NGS (Miseq platform) was used to sequence the env region of the HIV-1 quasispecies. Genetic distances were calculated, and phylogenetic trees were generated. We found both partners were infected with HIV-1 subtype circulating recombinant form (CRF), CRF65_cpx. The quasispecies distribution was relatively tightly clustered in the phylogenetic tree during early infection. Over time, the distribution of HIV-1 quasispecies gradually became more dispersed at 12 months, with a progressive increase in gene diversity. By 37 months, the sequences obtained for both partners formed different clusters in the phylogenetic tree. These results suggest that the HIV-1 contact tracing results generated by the Miseq platform may be more reliable than other conventional sequencing methods, which can provide important information about the transmission and evolution of HIV-1. Our findings may help to better target preventative interventions for promoting public health.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780463 | PMC |
| http://dx.doi.org/10.1038/s41598-018-19783-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Sensitivity and Consistency Comparison Between Next-Generation Sequencing and Sanger Sequencing in HIV-1 Pretreatment Drug Resistance Testing.
Viruses
October 2024
Department of HIV/STD Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Next-generation sequencing (NGS) for HIV drug resistance (DR) testing has an increasing number of applications for the detection of low-abundance drug-resistant variants (LA-DRVs) in regard to its features as a quasi-species. However, there is less information on its detection performance in DR detection with NGS. To determine the feasibility of using NGS technology in LA-DRV detection for HIV-1 pretreatment drug resistance, 80 HIV-infected individuals who had never undergone antiretroviral therapy were subjected to both NGS and Sanger sequencing (SS) in HIV-1 drug resistance testing.
View Article and Find Full Text PDFCompartmentalized HIV-1 reservoir in intestinal monocytes/macrophages on antiretroviral therapy.
J Infect Dis
November 2024
INSERM UMR1291-CNRS UMR5051-Université Toulouse III, Toulouse Institute for Infectious and Inflammatory Diseases F-31300, France.
Background: The persistence of latently infected cells prevents a cure of HIV. The intestinal mucosa contains numerous target cells, and high levels of HIV-1 DNA persist in this compartment under ART. While CD4+ T cells are the best characterized reservoir of HIV-1, the role of long-lived intestinal macrophages in HIV-1 persistence on ART remains controversial.
View Article and Find Full Text PDFClinical Pharmacology of the Single Tablet Regimen Bictegravir/Emtricitabine/Tenofovir Alafenamide in the evolving era of antiretroviral therapies.
New Microbiol
November 2024
Infectious Diseases Unit, Department of Medical Sciences, University of Turin, Italy.
In this fast-evolving era of antiretroviral chemotherapy, the single-tablet regimen (STR) BIC/FTC/TAF, an oral regimen including a potent INSTI (strand-transfer integrase inhibitors) like Bictegravir plus two different NRTIs (Nucleoside Reverse Transcriptase Inhibitors), is increasingly challenged by new oral combinations. Furthermore, long-acting injectable drugs have also been developed and others are being under development. Notably, no new STR consisting of two NRTIs plus a 3rd drug like an INSTI are in the industrial pipeline.
View Article and Find Full Text PDFThe relevance of ultradeep sequencing for low HIV-1 viral loads and proviruses in the clinical setting.
J Med Virol
August 2024
Laboratoire de Virologie, CHRU Nancy, Vandoeuvre-lès-Nancy, France.
Improving the therapeutic management of HIV-positive persons is a major public health issue and includes better detection of drug resistance mutations (DRMs). The aim of this study was (i) to explore DRMs in HIV-1-positive persons presenting a blood viral load (VL) < 1000 genomes copies (gc)/mL, including the analyze of cerebrospinal fluid (CSF) and HIV-DNA from peripheral blood mononuclear cells using ultradeep sequencing (UDS) and (ii), to evaluate how these DRMs could influence the clinical practices. For each patient (n = 12), including five low-VL patients (i.
View Article and Find Full Text PDFGenetic landscape for majority and minority HIV-1 drug resistance mutations in antiretroviral therapy naive patients in Accra, Ghana.
Heliyon
June 2024
Department of Medical Microbiology, Medical School, College of Health Sciences, University of Ghana, Accra, Ghana.
Article Synopsis
- - The study compared Sanger sequencing and next-generation sequencing (NGS) to detect drug-resistance mutations in HIV-1 among treatment-naive patients in Ghana, aiming to find minority mutations that could lead to treatment failure.
- - NGS identified 25 mutations in the HIV genes compared to 21 by Sanger sequencing, revealing a higher detection rate of minority drug resistance mutations (55% vs. 35%).
- - Results suggest NGS is more effective for accurate drug resistance testing and highlight the need for its implementation in clinical settings in Ghana, as Sanger sequencing alone may miss significant mutations.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!