Aim: To investigate the effects of combined use of emodin and baicalein (CEB) at the cellular and organism levels in severe acute pancreatitis (SAP) and explore the underlying mechanism.
Methods: SAP was induced by retrograde infusion of 5% sodium taurocholate into the pancreatic duct in 48 male SD rats. Pancreatic histopathology score, serum amylase activity, and levels of tumour necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and IL-10 were determined to assess the effects of CEB at 12 h after the surgery. The rat pancreatic acinar cells were isolated from healthy male SD rats using collagenase. The cell viability, cell ultrastructure, intracellular free Ca concentration, and inositol (1,4,5)-trisphosphate receptor (IPR) expression were investigated to assess the mechanism of CEB.
Results: Pancreatic histopathology score (2.07 ± 1.20 6.84 ± 1.13, < 0.05) and serum amylase activity (2866.2 ± 617.7 5241.3 ± 1410.0, < 0.05) were significantly decreased in the CEB (three doses) treatment group compared with the SAP group (2.07 ± 1.20 6.84 ± 1.13, < 0.05). CEB dose-dependently reduced the levels of the pro-inflammatory cytokines IL-6 (466.82 ± 48.55 603.50 ± 75.53, < 0.05) and TNF-α (108.04 ± 16.10 215.56 ± 74.67, < 0.05) and increased the level of the anti-inflammatory cytokine IL-10 (200.96 ± 50.76 54.18 ± 6.07, < 0.05) compared with those in the SAP group. CEB increased cell viability, inhibited cytosolic Ca concentration, and significantly ameliorated intracellular vacuoles and IP mRNA expression compared with those in the SAP group ( < 0.05). There was a trend towards decreased IPR protein in the CEB treatment group; however, it did not reach statistical significance ( > 0.05).
Conclusion: These results at the cellular and organism levels reflect a preliminary mechanism of CEB in SAP and indicate that CEB is a suitable approach for SAP treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757123 | PMC |
| http://dx.doi.org/10.3748/wjg.v24.i1.35 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect and safety of sivelestat on acute severe pancreatitis with systemic inflammatory response syndrome: a retrospective study.
Sci Rep
January 2025
Department of emergency ICU, the first affiliated hospital of Zhengzhou University, Henan, 450052, China.
The study was to explore the efficacy and safety of sivelestat (SV) in the treatment of severe acute pancreatitis (SAP) with systemic inflammatory response syndrome (SIRS). A total of 102 SAP patients diagnosed and treated in the Emergency Intensive Care Unit of the First Affiliated Hospital of Zhengzhou University from January 2021 to August 2024 were selected. The changes of disease outcome, hospital stays and mortality were compared between the two groups.
View Article and Find Full Text PDFProportional Assist Ventilation for Minimizing the Duration of Mechanical Ventilation (the PROMIZING study): update to the statistical analysis plan for a randomized controlled trial.
Trials
December 2024
Department of Critical Care, Keenan Research Centre, St Michael's Hospital, and Interdepartmental Division of Critical Care Medicine, University of Toronto, Toronto, Canada.
Background: We previously published the protocol and statistical analysis plan for a randomized controlled trial of Proportional Assist Ventilation for Minimizing the Duration of Mechanical Ventilation: the PROMIZING study in Trials ( https://doi.org/10.1186/s13063-023-07163-w ).
View Article and Find Full Text PDFDiscovery of novel bicyclic and tricyclic cyclohepta[b]thiophene derivatives as multipotent AChE and BChE inhibitors, in-Vivo and in-Vitro assays, ADMET and molecular docking simulation.
Eur J Med Chem
December 2024
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sinai University, Kantara Branch, Ismailia, 41636, Egypt.
Alzheimer's disease (AD) is primarily caused by oxidative stress, hyperphosphorylated τ-protein aggregation, and amyloid-β deposition. Changes in dopaminergic and serotoninergic neurotransmitter pathways are linked to certain symptoms of AD. Derivatives of bicyclic and tricyclic cyclohepta[b]thiophene were developed to identify new potential candidates as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors for the treatment of AD.
View Article and Find Full Text PDFImpact of race and ethnicity on glaucoma progression detection by perimetry and optical coherence tomography.
Sci Rep
December 2024
Bascom Palmer Eye Institute, University of Miami, 900 NW 17th St, Miami, FL, USA.
This study assessed the impact of race and ethnicity on longitudinal test variability and time to detect glaucoma progression using standard automated perimetry (SAP) and optical coherence tomography (OCT). The sample consisted of 47,003 SAP tests from 5402 eyes and 25,480 OCT tests from 4125 eyes, with 20% of participants self-identifying as Black or African American and 80% as White; 29% as Hispanic or Latino and 71% as Not Hispanic or Latino. Variability was measured using standard deviations of residuals from linear regression models for SAP mean deviation (MD) and OCT retinal nerve fiber layer (RNFL) thickness over time.
View Article and Find Full Text PDFAntibiofilm Activity of Epinecidin-1 and Its Variants Against Drug-Resistant and Isolates from Vaginal Candidiasis Patients.
Infect Dis Rep
December 2024
Microbial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli 620024, India.
Indwelling intrauterine contraceptive devices (IUDs) have surfaces that facilitate the attachment of spp., creating a suitable environment for biofilm formation. Due to this, vulvovaginal candidiasis (VVC) is frequently linked to IUD usage, necessitating the prompt removal of these devices for effective treatment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!