AI Article Synopsis
- - This paper introduces a method called Controlled Breakdown (CBD) for creating multiple nanopores in a silicon nitride membrane, allowing researchers to manage both how many nanopores are made and their sizes.
- - The study reveals that, even though the process is somewhat random, the nanopores produced tend to be uniform in diameter, which can be influenced by the duration of exposure and the strength of the electric field used.
- - The authors suggest that this approach simplifies fabrication, making it faster and less complicated than existing methods, with the potential to be integrated into optical systems for efficient testing and analysis.
Article Abstract
This paper reports a controlled breakdown (CBD) method to fabricate multiple nanopores in a silicon nitride (SiN) membrane with control over both nanopore count and nanopore diameter. Despite the stochastic process of the breakdown, we found that the nanopores created via CBD, tend to be of the same diameter. We propose a membrane resistance model to explain and control the multiple nanopores forming in the membrane. We prove that the membrane resistance can reflect the number of nanopores in the membrane and that the diameter of the nanopores is controlled by the exposure time and strength of the electric field. This controllable multiple nanopore formation via CBD avoids the utilization of complicated instruments and time-intensive manufacturing. We anticipate CBD has the potential to become a nanopore fabrication technique which, integrated into an optical setup, could be used as a high-throughput and multichannel characterization technique.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775244 | PMC |
| http://dx.doi.org/10.1038/s41598-018-19450-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
On the ability to extract MLVA profiles of Vibrio cholerae isolates from WGS data generated with Oxford Nanopore Technologies.
BMC Res Notes
January 2025
Center for Applied Molecular Technologies (CTMA), Institute of Clinical and Experimental Research (IREC), Université catholique de Louvain (UCLouvain), Brussels, Belgium.
Objective: Multiple-Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) is widely used to subtype pathogens causing foodborne and waterborne disease outbreaks. The MLVAType shiny application was previously designed to extract MLVA profiles of Vibrio cholerae isolates from whole-genome sequencing (WGS) data, and provide backward compatibility with traditional MLVA typing methods. The previous development and validation work was conducted using short (pair-end 300 and 150 nt long) reads from Illumina MiSeq and Hiseq sequencing.
View Article and Find Full Text PDFComplete genome sequence of subsp. BI040, a probiotic strain with multiple health benefits from the NORDBIOTIC collection.
Microbiol Resour Announc
January 2025
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
The complete genome of subsp. BI040-a human stool isolate, was sequenced using Illumina and Oxford Nanopore technologies. The BI040 genome is composed of a circular 1,944,141-bp chromosome which carries genes potentially involved in vitamin synthesis and gut health.
View Article and Find Full Text PDFNanopore Discriminates Watson-Crick and Hoogsteen Hydrogen Bonds in Multiple DNA Contexts.
Nano Lett
January 2025
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Watson-Crick and Hoogsteen hydrogen bonds aid the formation of highly ordered structures in genomic DNA that dynamically govern genetic modes such as gene regulation and replication. Hence, measuring and distinguishing these two types of hydrogen bonds in different DNA contexts are essential for understanding DNA architectures. However, due to their transient nature and minimal structure differences at the sub-nanometer scale, differentiating Watson-Crick hydrogen bonds from Hoogsteen hydrogen bonds is difficult.
View Article and Find Full Text PDFThe fly route of extended-spectrum-β-lactamase-producing dissemination in a cattle farm: from the ecosystem to the molecular scale.
Front Antibiot
April 2024
Transmission, Reservoir and Diversity of Pathogens Unit, Institut Pasteur, Les Abymes, France.
Introduction: This study aimed to understand the origin and to explain the maintenance of extended-spectrum β-lactamase (ESBL) isolated from food-producing animals in a third-generation cephalosporin (3GC)-free farm.
Methods: Culture and molecular approaches were used to test molecules other than 3GC such as antibiotics (tetracycline and oxytetracycline), antiparasitics (ivermectin, flumethrin, fenbendazol, and amitraz), heavy metal [arsenic, HNO, aluminum, HNO, cadmium (CdSO), zinc (ZnCl), copper (CuSO), iron (FeCl), and aluminum (AlSO)], and antioxidant (butylated hydroxytoluene) as sources of selective pressure. Whole-genome sequencing using short read (Illumina™) and long read (Nanopore™) technologies was performed on 34 genomes.
Mixed nontuberculous mycobacteria in an immunocompromised patient with probable progressive multifocal leukoencephalopathy.
IJID Reg
March 2025
SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Human Genetics, Stellenbosch University, Cape Town, South Africa.
Objectives: Nontuberculous mycobacteria (NTM) are increasingly recognized opportunistic pathogens found ubiquitously in the environment. The presence of multiple NTM species at the site of disease complicates diagnosis and treatment.
Case And Management: A 40-year-old patient who tested positive for HIV, with an absolute clusters of differentiation 4+ T-cell count of 3 cells/µl and cryptococcaemia, presented with hemoptysis, productive cough, and weight loss.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!