Characterisation of peptides in silages made from perennial ryegrass with different silage additives.

The effects of applying either formic acid (5.4 g kg ), a mixture of formic acid (2.7 g kg ) and formaldehyde (1.5 g kg , 81 g kg herbage crude protein) or two concentrations of a cysteine peptidase inhibitor, cystamine (5 or 50 g kg ), to perennial ryegrass (Lolium perenne) on the nitrogen (N) distribution of the resulting silages were investigated, with emphasis on changes in concentration, composition and molecular weight of silage peptides. Herbage (156 g dry matter kg and 141 g water-soluble carbohydrate kg dry matter) was ensiled in triplicate in laboratory silos for 100 days. Formic acid and the formic acid/formaldehyde mixture reduced soluble non-protein N and ammonia N concentrations (P < 0.01); in addition, formic acid increased peptide N concentrations (P < 0.05). Cystamine at 50 g kg reduced ammonia N concentrations (P < 0.01) and increased peptide N concentrations (P < 0.05), but when applied at 5 g kg had little effect. Gel filtration of silage extracts on Sephadex G-25 suggested that a small proportion (0.06-0.11 g kg peptide N) of silage peptides had a chain length of 7-9 amino acids, but remaining peptides were smaller with chain lengths of 2-6 amino acid residues. Amino acid analysis of silage peptides indicated that additive treatment had little effect on peptide amino acid composition but that peptides with a chain length of 7-9 amino acids contained lower proportions of isoleucine and arginine. © 2000 Society of Chemical Industry.