Oxysophoridine (OSR) is a major active alkaloid extracted from L. The aim of the present study was to investigate the induction of the apoptotic effects of OSR on colorectal cancer cells and . The results of the MTT and colony formation assays demonstrated that the proliferation of HCT116 cells was inhibited by OSR . The characteristics of cellular apoptosis in OSR-treated HCT116 cells were analyzed by Hoechst 33258 staining. It was also observed that the expression of caspase-3, B-cell lymphoma-2 (Bcl-2) associated X protein (Bax) and cytochrome c increased significantly upon OSR treatment. However, the expression of Bcl-2 and poly ADP-ribose polymerase-1 (PARP-1) was downregulated in OSR-treated cells compared with untreated cells. The experiments identified that OSR significantly inhibited the growth of the transplanted mouse CT26 tumor tissue, upregulated the expression of caspase-3, Bax and cytochrome c and downregulated the expression of Bcl-2 and PARP-1, as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. It may be concluded that OSR significantly induced apoptotic effects on colorectal cancer cells and , and that its mechanism may be associated with the Bcl-2/Bax/caspase-3 signaling pathway.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755128 | PMC |
http://dx.doi.org/10.3892/ol.2017.7227 | DOI Listing |
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