AI Article Synopsis
- - The study aimed to find an efficient way to decellularize bovine pericardia, which involves removing cells while preserving the extracellular matrix and ensuring biocompatibility for potential use in heart tissue engineering.
- - Various decellularization methods were tested, with freeze-thaw cycles combined with SDS and sodium deoxycholate (FTS) found to be the most effective at eliminating DNA and potential xenoantigens while maintaining collagen levels.
- - The FTS protocol demonstrated excellent preservation of the extracellular matrix, minimal immune response in animal tests, and no significant toxicity to human cells, indicating its potential as a scaffold for heart tissue applications.
Article Abstract
Objectives: In this study, we sought to explore an efficient decellularization protocol for bovine pericardia with better extracellular matrix preservation and good biocompatibility.
Methods: Bovine pericardia were decellularized by sodium dodecyl sulphate (SDS), SDS + sodium deoxycholate (SD), Triton X-100 (TX), TX + SD (TS), freeze-thaw cycles + SDS + SD (FSS) and freeze-thaw cycles + TX + SD (FTS), respectively. Untreated pericardia were used as native control. Histological examination, residual cellular content analysis, biochemical and biomechanical evaluations and cytotoxicity assay were performed to investigate decellularization efficiency, xenoantigens removal, extracellular matrix preservation and biocompatibility. In vivo biocompatibility was evaluated using a subcutaneous implantation method in rats.
Results: Among these protocols, FSS and FTS protocols were the most effective methods to remove both the DNA material and the galactose-α-1,3-galactose antigen. TX, TS and FTS bovine pericardia maintained the collagen content and had no cytotoxicity to human umbilical vein endothelial cells. The contents of elastin and glycosaminoglycan were lost to different degrees after decellularization, with the highest content of preservation with TX, followed by TS and FTS. Consistently, no significant difference was found between native bovine pericardia and TX, TS or FTS bovine pericardia. In vivo, FTS implants had minimal infiltration of macrophages and T-lymphocytes, with no histological evidence of peri-implant necrosis and calcification.
Conclusions: These results suggested that the FTS protocol showed optimal decellularization results with better extracellular matrix preservation and good biocompatibility. It may be a suitable protocol for producing a suitable scaffold for heart tissue engineering.
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| http://dx.doi.org/10.1093/icvts/ivx416 | DOI Listing |
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