Introduction: Rhodnius stali infection by Trypanosoma rangeli is reported in this study for the first time.
Methods: The triatomines were collected from the campus of the Federal University of Acre in Rio Branco, Acre, Brazil. The identification of T. rangeli was confirmed by multiplex polymerase chain reaction.
Results: The examinations of two specimens revealed R. stali infection by the epimastigote forms of T. rangeli.
Conclusions: The encounter of R. stali infected by T. rangeli generates an alert for the state of Acre, since the simultaneous presence with Trypanosoma cruzi can make the differential diagnosis of Chagas disease difficult.
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