AI Article Synopsis
- The study compared two extraction methods for cell wall-bound phenolics from maize tissues, focusing on efficiency and cost.
- Method 1 took longer (6 days) and was more expensive, using a solid-phase extraction cartridge, while Method 2 was quicker (2 days) and cost-effective, utilizing ethyl acetate.
- Both methods were found to be reliable, with Method 1 extracting more 4-coumarate and Method 2 yielding more ferulate, making Method 2 a preferred choice for large-scale plant breeding research.
Article Abstract
We compared two methods with different sample pretreatment, hydrolysis, and separation procedures to extract cell wall-bound phenolics. The samples were pith and rind tissues from six maize inbred lines reportedly containing different levels of cell wall-bound phenolics. In method 1, pretreated samples were extracted with a C solid-phase extraction cartridge, and it took 6 days to complete. In method 2, phenolics were extracted from crude samples with ethyl acetate, it took 2 days to complete, and the cost per sample was reduced more than 60%. Both methods extracted more 4-coumarate than ferulate. Overall, method 1 yielded more 4-coumarate, while method 2 yielded more ferulate. The lack of a genotype × method interaction and significant correlations between the results obtained using the two methods indicate that both methods are reliable for use in large-scale plant breeding programs. Method 2, scaled, is proposed for general plant biology research.
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| http://dx.doi.org/10.1021/acs.jafc.7b05752 | DOI Listing |
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