AI Article Synopsis
- Gliomas are the most common and deadly type of brain tumors, with a median survival rate of about one year, depending on genetic mutations.
- Novel imaging techniques like luciferase bioluminescence, micro-MRI, micro-CT, and micro-PET enhance research capabilities to study gliomas, particularly focusing on hypoxia, an important factor in tumor growth and drug resistance.
- The study outlines a method for injecting luciferase-expressing glioma cells into rat brains, utilizing various imaging tests to track tumor growth and assess the effects of treatments and genetic variations.
Article Abstract
Gliomas are the most common type of primary, malignant brain tumor and significantly impact patients, who have a median survival of ~1 year depending on mutational background. Novel imaging modalities such as luciferase bioluminescence, micro-magnetic resonance imaging (micro-MRI), micro-computerized tomography (micro-CT), and micro-positron emission tomography (micro-PET) have expanded the portfolio of tools available to study this disease. Hypoxia, a key oncogenic driver of glioma and mechanism of resistance, can be studied in vivo by the concomitant use of noninvasive MRI and PET imaging. We present a protocol involving stereotactic injection of syngenic F98 luciferase-expressing glioma cells generated by our laboratory into Fischer 344 rat brains and imaging using luciferase. In addition, 18-F-fludeoxyglucose, 18F-fluoromisonidazole, and 18F-fluorothymidine PET imaging are compared with quantified luciferase flux. These tools can potentially be used for assessing tumor growth characteristics, hypoxia, mutational effects, and treatment effects.
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| http://dx.doi.org/10.1007/978-1-4939-7665-2_26 | DOI Listing |
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