A technique of ultrasonically guided percutaneous puncture of Wirsung's duct for pancreatic juice collection and radiological opacification is described. The equipment used is a linear array real-time transducer with a groove into which a metallic support provided with a guiding needle is attached. Once the depth at which the duct lies has been determined, a Chiba's needle is passed through the guiding needle and pushed up to the corresponding length. Pure pancreatic juice is then withdrawn for lactoferrin assay and a contrast medium is injected for pancreatography.

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