AI Article Synopsis
- Chronic renal allograft loss is typically a gradual process, and this study aimed to explore biomarkers for early detection.
- The researchers examined the expression of EMT markers (vimentin and β-catenin) and measured specific mRNA levels in urinary cells of 75 renal transplant patients.
- Findings indicated a correlation between vimentin and CD45 mRNA levels and vimentin immunostaining scores, suggesting these markers could serve as a noninvasive method for monitoring kidney transplant health and identifying fibrotic disease early.
Article Abstract
Renal allograft loss is most often a chronic process, irrespective of the mechanism at stake. In this prospective study, we studied the expression of epithelial to mesenchymal transition (EMT) markers vimentin and β-catenin by immunohistochemistry in the surveillance biopsy and measured the mRNA encoding vimentin (VIM), CD45, GAPDH and uroplakin 1a (UPK) by quantitative PCR in urinary cells in 75 renal transplant patients. The aim is to establish a simple screening test for chronic renal allograft dysfunction. We found that the value of the mRNA of vimentin and CD45 relative to the uroplakin 1a (UPK) mRNA is correlated with the score in vimentin immunostaining in routine biopsies. These biomarkers could be used as a noninvasive tool to monitor the renal graft fibrogenesis. This test could be used for early detection of fibrotic diseases of the kidney transplant.
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| http://dx.doi.org/10.1016/j.nephro.2017.09.002 | DOI Listing |
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