AI Article Synopsis
- The transcription factor Sox2 is crucial for determining the fate of pluripotent and neural stem cells, and its levels need to be precisely regulated.
- The study reveals that the long non-coding RNA Sox2ot negatively regulates Sox2 levels during the differentiation of mouse embryonic stem cells, meaning when Sox2ot levels go up, Sox2 levels go down.
- The researchers found that increased Sox2ot transcription is linked to reduced Sox2 RNA levels and less interaction with regulatory elements that enhance Sox2 expression, suggesting a specific mechanism at play during development.
Article Abstract
The transcription factor Sox2 controls the fate of pluripotent stem cells and neural stem cells. This gatekeeper function requires well-regulated Sox2 levels. We postulated that Sox2 regulation is partially controlled by the Sox2 overlapping long non-coding RNA (lncRNA) gene Sox2ot. Here we show that the RNA levels of Sox2ot and Sox2 are inversely correlated during neural differentiation of mouse embryonic stem cells (ESCs). Through allele-specific enhanced transcription of Sox2ot in mouse Sox2eGFP knockin ESCs we demonstrate that increased Sox2ot transcriptional activity reduces Sox2 RNA levels in an allele-specific manner. Enhanced Sox2ot transcription, yielding lower Sox2 RNA levels, correlates with a decreased chromatin interaction of the upstream regulatory sequence of Sox2 and the ESC-specific Sox2 super enhancer. Our study indicates that, in addition to previously reported in trans mechanisms, Sox2ot can regulate Sox2 by an allele-specific mechanism, in particular during development.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762901 | PMC |
| http://dx.doi.org/10.1038/s41598-017-18649-4 | DOI Listing |
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