AI Article Synopsis
- The study investigated the antibacterial effectiveness of a specific porphyrin compound against four types of multidrug-resistant bacteria: Staphylococcus aureus and Enterococcus faecalis (Gram-positive), and Escherichia coli and Pseudomonas aeruginosa (Gram-negative).
- Using a concentration of 10 μg/ml of the porphyrin and a 60-second light exposure, the researchers found that S. aureus and E. faecalis were highly susceptible to the treatment, while E. coli and P. aeruginosa showed much less susceptibility.
- When Gram-negative bacteria were pre-treated with EDTA before photo-activation, there was a significant reduction in their viable counts, suggesting that this method could be a promising alternative
Article Abstract
The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable susceptibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli and P. aeruginosa showed very low susceptibility. In a later stage, suspensions of Gram-negative bacteria were processed with EDTA before photo-activation, obtaining a significant decrease in viable counts. In view of the results, if the combination of low porphyrin concentrations and short irradiation times will be effective in vivo also, this approach could be a possible alternative to antibiotics, in particular against localized infections due to multidrug-resistant microorganisms.
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| http://dx.doi.org/10.5604/01.3001.0010.7092 | DOI Listing |
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