AI Article Synopsis
- The main challenge in cancer gene therapy is finding effective delivery methods that protect the genes from degradation and minimize harmful side effects.
- PEGylated liposomes have shown promise in delivering small molecule drugs and may be effective for nucleic acid delivery, thanks to their PEG coating which helps avoid immune response and enhance circulation.
- This study focused on using PEGylated liposomes to deliver small interfering RNA (siRNA) for silencing genes associated with human papillomavirus (HPV) and cervical cancer, emphasizing the importance of targeting peptides and polymer complexation for successful delivery.
Article Abstract
The greatest obstacle to clinical application of cancer gene therapy is lack of effective delivery tools. Gene delivery vehicles must protect against degradation, avoid immunogenic effects and prevent off target delivery which can cause harmful side effects. PEGylated liposomes have greatly improved tumor localization of small molecule drugs and are a promising tool for nucleic acid delivery as the polyethylene glycol (PEG) coating protects against immune recognition and blood clearance. In this study, small interfering RNA (siRNA) was fully encapsulated within PEGylated liposomes by complexing the siRNA with a cationic polymer, polyethyleneimine (PEI), before encapsulation. Formation methods and material compositions were then investigated for their effects on encapsulation. This technology was translated for protective delivery of siRNA designed for human papillomavirus (HPV) viral gene silencing and cervical cancer treatment. PEGylated liposomes encapsulating siRNA were functionalized with the AG86 targeting peptide-amphiphile which binds to the αβ integrin, a cervical cancer biomarker. It was found that both targeting and polymer complexation before encapsulation were critical components to effective transfection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675078 | PMC |
| http://dx.doi.org/10.1002/btm2.10022 | DOI Listing |
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