Association between polymorphisms and the risk of inflammatory bowel disease in the Polish population.

Purpose: The main aim of this study was investigate the association between the genetic polymorphism of antioxidant enzyme genes: and and the risk of inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis in the Polish population.

Methods: A total of 445 subjects including 200 patients with IBD and 245 controls were allowed in this study. We determined activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx1) and examination their association with the SNPs of respective genes ( +35A/C, C-262T and Pro197Leu). RFLP technique was used to determine the selected genes polymorphisms. Antioxidant enzymes activity were evaluated in erythrocyte hemolysate of 23 patients with non-active IBD and 30 healthy participants.

Results: The A/C genotype and the C allele frequencies of A/C polymorphism of gene were significantly associated with the reduced risk of IBD (OR=0.43; 95% CI 0.23; 0.83). Alike, C/T (OR=0.45; 95% CI= 0.29; 0.70) and T/T genotype (OR=0.43; 95% CI= 0.21; 0.87) of gene polymorphism diminished the susceptibility to IBD. A significant decrease of CAT (=0.028) and increase of GPx1 (=0.025) enzyme activities were seen in IBD patients compared to control.

Conclusions: Our data confirm dysregulated antioxidant capacity in patients suffering from IBD. Both, the A/C genotype as well as C/T and T/T genotypes may be associated with a reduction risk of IBD in the Polish population.