AI Article Synopsis
- This study investigates how IL-25 affects mesenchymal stem cells' (MSCs) ability to help regenerate intestinal epithelial cells, particularly in inflammatory bowel disease (IBD) patients.
- It involved analyzing specific cell types in patients with Crohn's disease and ulcerative colitis, using techniques such as immunofluorescence staining and flow cytometry, as well as testing how MSCs treated with IL-25 influence cell growth.
- The findings indicate that IL-25R plays a role in mucosal inflammation and cell proliferation in IBD, suggesting that MSC therapy enhanced by IL-25 could be a promising new treatment approach for these conditions.
Article Abstract
Background: This study aimed to investigate the influence of IL-25 on the capacity of mesenchymal stem cells (MSCs) to induce intestinal epithelial cell regeneration.
Methods: The CD4IL-25R cells and LGR5IL-25R cells in colonic mucosa of Crohn's disease (CD) patients, ulcerative colitis (UC) patients and healthy controls were detected by immunofluorescence staining, and the CD4IL-25R cells in peripheral blood were detected by flow cytometry. Rat MSCs were separated and stimulated with IL-25. Then, MSCs were further incubated in IL-25-free DMEM for 24 h, and this DMEM was collected as conditioned medium (CM). IEC-6 cells were divided into 3 groups: experimental group (CM and TNF-α), control group (DMEM and TNF-α) and negative control group (DMEM).
Results: The CD4IL-25R cells and LGR5IL-25R cells significantly increased in the colonic mucosa of active CD patients and UC patients compared with IBD patients in remission and healthy controls. The CD4IL-25R cells reduced in peripheral blood of IBD patients, which was inversely correlated with inflammatory markers (ESR and CRP). CM facilitated the migration and proliferation of IEC-6 cells in the presence of TNF-α. The protein expression of AKT, p38 and ERK increased in IEC-6 cells after treatment with CM and TNF-α.
Conclusion: IL-25R is involved in Th-related mucosal inflammation and proliferation of intestinal stem cells in IBD. IL-25 enhances the capacity of MSC to induce intestinal epithelial cell regeneration, and MSC therapy with IL-25 may be a new direction for IBD treatment.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752884 | PMC |
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