In this study, described an electrochemical immunoassay for insulin that is based on the use of zinc silicate spheres loaded with palladium nanoparticles (ZnSiO-PdNPs) that act as dual-function labels. The ZnSiO-PdNPs display high electrocatalytic activity towards the reduction of HO and high sensitivity in chronoamperometry. The ZnSiO-PdNPs decrease the electron transfer rate between the electrolyte and the surface of the electrode, which can increase the changed current and enhance the sensitivity of the immunosensor as detected by square wave voltammetry (SWV). Electrodeposited gold is used as the matrix material. The icosahedral gold nanocrystals are coated with the primary antibodies formed a 3D mode to against abundant of insulin. Under optimal conditions, the assay has a linear response in the 0.1pgmL to 50ngmL insulin concentration range, and the limit of detection of the SWV and CA methods are 0.25 fg mL and 80 fg mL, respectively. Moreover, the immunosensor holds an outstanding analytical performance for the insulin detection and has promising potential in clinical diagnosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.talanta.2017.11.037 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Emirates consensus recommendations on cardiovascular risk management in type 2 diabetes.
Front Endocrinol (Lausanne)
January 2025
Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.
Background: The combination of cardiovascular disease and diabetes is a highly prevalent condition in the United Arab Emirates. Development and dissemination of evidence-based regional recommendations for optimal screening, treatment and referrals of people with diabetes and high cardiovascular risk is an important priority.
Consensus Panel: An expert panel of diabetologists, endocrinologists and cardiologists from the Emirates Cardiac Society and Emirates Diabetes and Endocrine Society as well as different entities in the UAE, discussed and reviewed evidence and also a consensus report from the American Diabetes Association to formulate contextualized recommendations that could be applied for optimal management of cardiovascular risk in people with diabetes in the UAE.
Bile acids and incretins as modulators of obesity-associated atherosclerosis.
Front Cardiovasc Med
January 2025
Division of Physiology and Pathophysiology, Otto Loewi Research Center for Vascular Biology, Immunology and Inflammation, Medical University of Graz, Graz, Austria.
Obesity is one of the major global health concerns of the 21st century, associated with many comorbidities such as type 2 diabetes mellitus (T2DM), metabolic dysfunction-associated steatotic liver disease, and early and aggressive atherosclerotic cardiovascular disease, which is the leading cause of death worldwide. Bile acids (BAs) and incretins are gut hormones involved in digestion and absorption of fatty acids, and insulin secretion, respectively. In recent years BAs and incretins are increasingly recognized as key signaling molecules, which target multiple tissues and organs, beyond the gastro-intestinal system.
View Article and Find Full Text PDFAssociation between triglyceride-glucose (TyG) index and risk of depression in middle-aged and elderly Chinese adults: Evidence from a large national cohort study.
Biomol Biomed
January 2025
Department of Stress Medicine, Faculty of Psychology, Second Military Medical University, Shanghai, China.
Insulin resistance (IR) has been proposed as a contributing factor to major depressive disorder (MDD), with previous studies reporting a positive correlation between triglyceride-glucose (TyG) a proxy indicator of IR and MDD. However, limited information is available regarding their longitudinal association. This study aimed to clarify the connection between TyG levels and depression risk, as well as explore its predictive potential.
View Article and Find Full Text PDFExploring the prevalence of gout among underrepresented low socioeconomic status type 2 diabetes populations.
Diabetol Metab Syndr
January 2025
Philadelphia College of Osteopathic Medicine, Philadelphia, USA.
Background: Underserved and underrepresented populations often lack access to affordable, quality healthcare, educational resources, and nutritious foods, all of which contribute to increased risk of Type 2 Diabetes and gout. Type 2 Diabetes is a condition characterized by the denaturation of the insulin receptors, due to chronically high blood glucose levels, leading to impaired regulation of blood sugar. Gout is a chronic inflammatory disease affecting joints in the lower limbs, marked by elevated serum urate levels and the accumulation of uric acid crystals in synovial fluid, causing painful flare-ups that significantly impact quality of life.
View Article and Find Full Text PDFTargeting GABA signaling in type 1 diabetes and its complications- an update on the state of the art.
Pharmacol Rep
January 2025
Faculty of Pharmaceutical Sciences, Medical University of Silesia in Katowice, Jedności 8, Sosnowiec, 41-200, Poland.
Type 1 diabetes (T1D) is an autoimmune disease that leads to the progressive destruction of insulin-producing β cells, resulting in lifelong insulin dependence and a range of severe complications. Beyond conventional glycemic control, innovative therapeutic strategies are needed to address the underlying disease mechanisms. Recent research has highlighted gamma-aminobutyric acid (GABA) as a promising therapeutic target for T1D due to its dual role in modulating both β cell survival and immune response within pancreatic islets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!