The effects of water on the structure and dynamics of natural and regenerated silk fibroin (SF) samples were studied using C solid-state nuclear magnetic resonance (NMR) spectroscopy. We prepared different types of SF materials, sponges, and fibers with different preparation methods and compared their NMR spectra in the dry and hydrated states. Three kinds of C NMR techniques, r-INEPT, CP/MAS, and DD/MAS, coupled with C isotope labeling of Ser, Tyr, and Ala residues were used. In the hydrated sponges, several conformations, that is, Silk I* and two kinds of β-sheets, A and B, random coil, and highly mobile hydrated random coil were observed, and the fractions were determined. The fractions were remarkably different among the three sponges but with only small differences among the regenerated and native fibers. The increase in the fraction of β-sheet B might be one of the structural factors for preparing stronger regenerated SF fiber.
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| http://dx.doi.org/10.1021/acs.biomac.7b01665 | DOI Listing |
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