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Prevalence of Gastrointestinal Parasites in Zoo Animals and Phylogenetic Characterization of (Linstow, 1902) and (Rudolphi, 1819) in Jiangsu Province, Eastern China.
Animals (Basel)
January 2024
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Article Synopsis
- * Results indicate a 42.3% overall infection rate, with higher rates found in non-primate mammals (50.0%) compared to birds (26.3%) and primates (31.6%).
- * A total of 11 parasite species were identified, highlighting potential zoonotic risks, emphasizing the necessity for improved parasite management to ensure the welfare of both animals and human visitors in the zoo.
Isolation of stage-specific spermatogenic cells by dynamic histone incorporation and removal in spermatogenesis.
Cytometry A
April 2024
Institute of Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
Due to the lack of an efficient in vitro spermatogenesis system, studies on mammalian spermatogenesis require the isolation of specific germ cell populations for further analyses. BSA gradient and elutriation have been used for several decades to purify testicular germ cells; more recently, flow cytometric cell sorting has become popular. Although each method has its advantages and disadvantages and is used depending on the purpose of the experiment, reliance on flow cytometric cell sorting is expected to be more prevalent because fewer cells can be managed.
View Article and Find Full Text PDFManufacturing and validation of Good Manufacturing Practice-compliant regulatory dendritic cells for infusion into organ transplant recipients.
Cytotherapy
April 2023
Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Clinical and Translational Science Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. Electronic address:
Background Aims: Regulatory (or "tolerogenic") dendritic cells (DCregs) are a highly promising, innovative cell therapy for the induction or restoration of antigen-specific tolerance in immune-mediated inflammatory disorders. These conditions include organ allograft rejection, graft-versus-host disease following bone marrow transplantation and various autoimmune disorders. DCregs generated for adoptive transfer have potential to reduce patients' dependence on non-specific immunosuppressive drugs that can induce serious side effects and enhance the risk of infection and certain types of cancer.
View Article and Find Full Text PDFTranscriptome analysis of the binucleate ciliate with asynchronous nuclear cell cycles.
Mol Biol Cell
February 2023
Department of Cell Biology and Genetics, Texas A&M University Health Science Center, College Station, TX 77840.
harbors two functionally and physically distinct nuclei within a shared cytoplasm. During vegetative growth, the "cell cycles" of the diploid micronucleus and polyploid macronucleus are offset. Micronuclear S phase initiates just before cytokinesis and is completed in daughter cells before onset of macronuclear DNA replication.
View Article and Find Full Text PDFSynchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation.
Methods Mol Biol
September 2022
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, UK.
Centrifugal counter-flow elutriation is a non-invasive technique that separates cells based on their hydrodynamic volume in a specialized centrifugation chamber that allows the application of a counter-flow of buffer to oppose sedimentation. Here, we report a centrifugal counter-flow elutriation protocol for Trypanosoma brucei cells that is able to rapidly isolate highly enriched G1 subpopulations (>95%) of synchronized cells. The cells obtained are viable and proliferate without lag, allowing subsequent cell cycle phases to be obtained by continued culture.
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