We dekaryotized the multinucleate fungus Leucocoprinus gongylophorus, a symbiotic fungus cultivated vegetatively by leafcutter ants as their food. To track genetic changes resulting from dekaryotization (elimination of some nuclei from the multinuclear population), we developed two multiplex microsatellite fingerprinting panels (15 loci total), then characterized the allele profiles of 129 accessions generated by dekaryotization treatment. Genotype profiles of the 129 accessions confirmed allele loss expected by dekaryotization of the multinucleate fungus. We found no evidence for haploid and single-nucleus strains among the 129 accessions. Microscopy of fluorescently stained dekaryotized accessions revealed great variation in nuclei number between cells of the same vegetative mycelium, with cells containing typically between 3 and 15 nuclei/cell (average = 9.4 nuclei/cell; mode = 8). We distinguish four mycelial morphotypes among the dekaryotized accessions; some of these morphotypes had lost the full competence to produce gongylidia (nutritive hyphal-tip swellings consumed by leafcutter ants as food). In mycelial growth confrontations between different gongylidia-incompetent accessions, allele profiles suggest exchange of nuclei between dekaryotized accessions, restoring full gongylidia competence in some of these strains. The restoration of gongylidia competence after genetic exchange between dekaryotized strains suggests the hypothesis that complementary nuclei interact, or nuclear and cytoplasmic factors interact, to promote or enable gongylidia competence.
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http://dx.doi.org/10.1080/00275514.2017.1400304 | DOI Listing |
mBio
December 2024
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
Unlabelled: A novel Hendra virus (HeV) genotype (HeV genotype 2 [HeV-g2]) was recently isolated from a deceased horse, revealing high-sequence conservation and antigenic similarities with the prototypic strain, HeV-g1. As the receptor-binding (G) and fusion (F) glycoproteins of HeV are essential for mediating viral entry, functional characterization of emerging HeV genotypic variants is key to understanding viral entry mechanisms and broader virus-host co-evolution. We first confirmed that HeV-g2 and HeV-g1 glycoproteins share a close phylogenetic relationship, underscoring HeV-g2's relevance to global health.
View Article and Find Full Text PDFCurr Biol
November 2024
Ecologie Systématique Evolution, CNRS, Université Paris-Saclay, AgroParisTech, 91190 Gif-sur-Yvette, France. Electronic address:
Eukaryotes evolved from prokaryotic predecessors in the early Proterozoic and radiated from their already complex last common ancestor, diversifying into several supergroups with unresolved deep evolutionary connections. They evolved extremely diverse lifestyles, playing crucial roles in the carbon cycle. Heterotrophic flagellates are arguably the most diverse eukaryotes and often occupy basal positions in phylogenetic trees.
View Article and Find Full Text PDFCureus
October 2024
Oral and Maxillofacial Surgery, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Trabecular juvenile ossifying fibroma (TrJOF) is a rare, benign, fibro-osseous lesion that predominantly affects children and adolescents. The lesion is locally aggressive, has a high recurrence rate, and is often misdiagnosed due to its clinical and radiographic similarities to other lesions. A seven-year-old female presented with a history of swelling and pain on the right side of her face for the last month.
View Article and Find Full Text PDFJ Investig Med High Impact Case Rep
November 2024
University Al-Qadisiyah, Diwaniya, Iraq.
Tuberculosis (TB), caused by , is a leading infectious disease with varied manifestations. We report a rare presentation of gastric TB in a 50-year-old immunocompetent woman from the Middle East with no prior medical history. The patient presented with persistent epigastric pain, weight loss, nausea, and vomiting over a 2-month duration.
View Article and Find Full Text PDFCells
October 2024
Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
The bi- or multinucleated Reed-Sternberg cell (RS) is the diagnostic cornerstone of Epstein-Barr Virus (EBV)-positive and EBV-negative classical Hodgkin lymphoma (cHL). cHL is a germinal center (GC)-derived B-cell disease. Hodgkin cells (H) are the mononuclear precursors of RS.
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