Premise Of The Study: The swamp orchid, (Orchidaceae), is nationally endangered due to illegal collection and habitat loss and fragmentation, resulting in a disjunct distribution in spring and coastal wetland ecotones along Australia's east coast. Polymorphic microsatellite markers were developed to study genetic diversity and population structure for conservation and restoration purposes.
Methods And Results: Illumina HiSeq high-throughput sequencing was used to develop 15 nuclear microsatellite markers, including 10 polymorphic markers for . Polymorphism at each marker was evaluated using 90 individuals from four natural populations. The number of alleles per locus ranged from one to three, and the observed and expected heterozygosity varied from 0.036 to 0.944 and from 0.035 to 0.611, respectively. These markers transferred successfully to congener .
Conclusions: The microsatellite markers will be useful for revealing levels of genetic diversity and gene flow for and may inform future conservation efforts.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5749820 | PMC |
| http://dx.doi.org/10.3732/apps.1700085 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tracking Somatic Mutations for Lineage Reconstruction.
Methods Mol Biol
January 2025
Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel.
The human genome is composed of distinct genomic regions that are susceptible to various types of somatic mutations. Among these, Short Tandem Repeats (STRs) stand out as the most mutable genetic elements. STRs are short repetitive polymorphic sequences, predominantly situated within noncoding sectors of the genome.
View Article and Find Full Text PDFDevelopment of specific molecular markers for medicinal peony () with double flower.
J Genet
January 2025
College of Life Science, Sichuan Agricultural University, Sichuan 625014, People's Republic of China.
In China, medicinal with double flowers (DFs) does not produce seeds, yet it possesses significantly higher paeoniflorin content compared with its single-flowered counterpart. The propagation of medicinal with DFs relies solely on rhizomes. However, due to economic motivations, the rhizomes of medicinal with single flowers (SFs) are often mixed with those of medicinal with DFs.
View Article and Find Full Text PDFIntratumoural CD8 CXCR5 follicular cytotoxic T cells have prognostic value and are associated with CD19 CD38 B cells and tertiary lymphoid structures in colorectal cancer.
Cancer Immunol Immunother
December 2024
Department of Clinical Laboratory, State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Background: Colorectal cancer (CRC) is the most common digestive cancer in the world. Microsatellite stability (MSS) and microsatellite instability (MSI-high) are important molecular subtypes of CRC closely related to tumor occurrence and progression and immunotherapy efficacy. The presence of CD8 CXCR5 follicular cytotoxic T (T) cells is strongly associated with autoimmune disease and CD8 effector function.
View Article and Find Full Text PDFFirst microsatellite markers for the Amazonian species Copaifera multijuga Hayne developed using next-generation sequencing.
Mol Biol Rep
December 2024
Laboratório de Biologia Molecular (LBM), Centro de Bionegócios da Amazônia (CBA), Manaus, Amazonas, Brazil.
Background: Native to the Amazon region, Copaifera multijuga Hayne is a large tree (≈ 36 m in height) that is heavily exploited for extraction of its oleoresin. Many studies have addressed the phytochemical properties and applications of this raw material; however, there are few initiatives that have focused on the genetic characterization of native populations of this species. To this end, our objective was to develop microsatellite markers for C.
View Article and Find Full Text PDFDevelopment of novel microsatellite marker sets for 5 common tropical hydrozoan species (Cnidaria: Hydrozoa) from the Southwestern Indian Ocean.
Mol Biol Rep
December 2024
Laboratoire d'Excellence CORAIL, Papetoai, Moorea, French Polynesia.
Background: In the context of global change, coral reefs and their associated biodiversity are under threat. Several conservation strategies using population genetics have been explored to protect them. However, some components of this ecosystem are understudied, such as hydrozoans, an important class of benthic organisms worldwide.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!