Complete genome sequence of Tsukamurella sp. MH1: A wide-chain length alkane-degrading actinomycete.

J Biotechnol

Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing 100871, PR China. Electronic address:

Published: February 2018

AI Article Synopsis

  • Tsukamurella sp. strain MH1 was isolated from petroleum-contaminated soil in Romania and is capable of using various n-alkanes as its sole carbon source.
  • The complete genome of MH1, which consists of a single circular chromosome with a G+C content of 71.12%, has been sequenced, revealing it is identified as Tsukamurella carboxydivorans.
  • This genome is the first complete genome of this species, providing valuable information on its potential for bioremediation of oil-contaminated sites and its role in the environment.

Article Abstract

Tsukamurella sp. strain MH1, capable to use a wide range of n-alkanes as the only carbon source, was isolated from petroleum-contaminated soil (Pitești, Romania) and its complete genome was sequenced. The 4,922,396 bp genome contains only one circular chromosome with a G + C content of 71.12%, much higher than the type strains of this genus (68.4%). Based on the 16S rRNA genes sequence similarity, strain MH1 was taxonomically identified as Tsukamurella carboxydivorans. Genome analyses revealed that strain MH1 is harboring only one gene encoding for the alkB-like hydroxylase, arranged in a complete alkane monooxygenase operon. This is the first complete genome of the specie T. carboxydivorans, which will provide insights into the potential of Tsukamurella sp. MH1 and related strains for bioremediation of petroleum hydrocarbons-contaminated sites and into the environmental role of these bacteria.

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http://dx.doi.org/10.1016/j.jbiotec.2017.12.013DOI Listing

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