In vitro fermentation of copra meal hydrolysate by chicken microbiota.

The aim of this study was to carry out preliminary investigations on the in vitro fermentation selectivity of copra meal hydrolysate (CMH) by chicken gut microbiota. The ileum and cecum contents from three 35-day-old birds were used as inocula. Yeast mannooligosaccharide (yeast-MOS) or α-mannan was selected as a positive control. Batch culture fermentation with fecal bacteria was performed at 42 °C for 24 h in an anaerobic chamber. Samples were collected at 0, 6, 12, 18 and 24 h of fermentation and evaluated using real-time PCR and short-chain fatty acid (SCFA) analysis. Results showed that the medium containing ileum and both CMH and yeast-MOS substrates led to an increase in the growth of the dominant groups as , Enterobacteriaceae and spp. compared with 0-h fermentation. spp. and spp. were not detected in any samples. A significant decrease in was observed in all substrates tested after 6 h of fermentation ( < 0.05). Only the sample from CMH fermentation showed a significantly greater reduction in the population of after 18-h fermentation with ileum content ( < 0.05). Propionate was the main fermentation product found in both ileum and cecum fermentation followed by lactate and acetate. CMH can be utilized by ileum and cecum microbial of chickens, and CMH has a generally desirable effect on the microbiota. CMH has the potential for use as a supplementary diet with similar or improved benefits and lower costs compared to commercial prebiotics. Further experiments in animal trials would seem to be justified.