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Robust Immobilization and Activity Preservation of Enzymes in Porous Frameworks by Silica-Based "Inorganic Glue".
Adv Mater
December 2024
MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, P. R. China.
The development of novel methods to enhance enzyme-carrier interactions in situ, at a feasible cost, and on a large scale is crucial for improving the stability and durability of current immobilized enzyme systems used in industrial settings. Here, a pioneering approach termed "silica-based inorganic glue" is proposed, which utilizes protein-catalyzed silicification to fix enzyme within porous matrix while preserving enzyme activity. This innovative strategy offers several key benefits, including conformational stabilization of enzymes, improved interactions between enzymes and the matrix, prevention of enzyme leakage, and mitigation of pore blocking.
View Article and Find Full Text PDFApplying Ra and Ra to Trace Lateral Groundwater Discharge into Lake Qinghai, China.
Ground Water
December 2024
Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510006, China.
Quantifying lacustrine groundwater discharge (LGD) is important for understanding the dynamics of lake ecosystems and their expansion. This study focuses on Lake Qinghai, employing radium isotope models to evaluate the contributions of both shallow and deep groundwater. The data indicate that the activity of Ra and Ra demonstrates a pronounced gradient, decreasing from the shoreline to the center of Lake Qinghai.
View Article and Find Full Text PDFEnhancing human ACE2 expression in mouse models to improve COVID-19 research.
FEBS Open Bio
December 2024
Guangzhou National Laboratory, Guangzhou, China.
Mice are one of the most common biological models for laboratory use. However, wild-type mice are not susceptible to COVID-19 infection due to the low affinity of mouse ACE2, the entry protein for SARS-CoV-2. Although mice with human ACE2 (hACE2) driven by Ace2 promoter reflect its tissue specificity, these animals exhibit low ACE2 expression, potentially limiting their fidelity in mimicking COVID-19 manifestations and their utility in viral studies.
View Article and Find Full Text PDFClinical Profile, Comorbidities and Therapies in Type 2 Diabetes Patients on Sitagliptin-Based Therapy in Indian Outpatient Setting.
Cureus
November 2024
Medical Affairs, Lupin Limited, Mumbai, IND.
Objectives The study was conducted to generate real-world data on prescription patterns and patient profiles for sitagliptin-based therapies in real-world outpatient settings across India. Method A cross-sectional, observational, multicenter, real-world prescription event monitoring (PEM) study was conducted at 1058 sites across India over six months, from 1 August 2023 to 16 January 2024. Adult type 2 diabetes patients receiving sitagliptin-based mono or combination therapies were included in the study.
View Article and Find Full Text PDFCorrigendum to "Transcriptomics reveals crowding stress inhibit the immune defense of the head kidney of the pearl gentian grouper juvenile through NF-κB signal pathway" (162), January 2025, 105299.
Dev Comp Immunol
December 2024
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, 266071, PR China; Mingbo Aquatic Products Co., Ltd, Yantai, 261400, PR China. Electronic address:
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